缺氧耐受形成中脑内一氧化氮合成酶及L-精氨酸的变化

Changes of nitric oxide synthetase and L-arginine in generation of hypoxia tolerance

目的 :探讨缺氧耐受形成中脑内一氧化氮 ( NO)减少的机理。方法 :采用高压液相 Acc Q·Tag柱前衍生法测定缺氧耐受形成中小鼠脑内 L-精氨酸含量 ,双波长分光光度法测定缺氧耐受形成中小鼠脑内一氧化氮合成酶 ( NOS)活性。结果 :L-精氨酸 :1次缺氧组与正常对照组比有升高的趋势 ,但无统计学意义 ( P>0 .0 5) ;2 ,3,4次缺氧组比 1次缺氧组明显下降 ( P<0 .0 5) ;2 ,3,4次缺氧组比正常对照组有下降的趋势 ,但无统计学意义 ( P>0 .0 5) ;1次缺氧组与正常对照组比NOS活性显著升高 ;4次缺氧组与 1次缺氧组比有下降趋势 ,但无统计学意义 ( P>0 .0 5)。结论 :脑内 L-精氨酸含量减少及 NOS活性增加的抑制 ,导致脑内 NO合成减少 ,促进了缺氧耐受性的形成。

砛 Objective:To study mechanism of nitric oxide (NO) decreasing in generation of hypoxia tolerance. Methods: NOS activity of mouse brain was detected with spectrometer and L Arginine of mouse brain,with HPLC in generation of hypoxia tolerance.Results:Contents of L ARG in exposure to hypoxia one time were higher than those in control group ( P >0 05) and the contents in exposure to hypoixa 2,3 and 4 time were lower than those in 1 group ( P <0 05).And that of exposure to hypoxia 2,3 and 4 times was lower than that in control group ( P >0 05).NOS activity of mouse brain tissues in exprosure to hypoxia one time was higher than that in control group ( P <0 05).Whole NOS activity in exposure to hypoxia 4 times tended to decrease.Conclusion:These results indicated that decreasing of L arginine and inhibition of NOS activity increasing could lead to the decrease of NO synthesis in mouse brain and enhance forming of acute hypoxidc tolerance in mice. 〔