电离辐射对小鼠腹腔巨噬细胞POMC转录水平的影响

Effect of irradiation on POMC transcription level in peritoneal macrophages of mice

目的 :观察 75m Gy、2 .0 Gy X射线全身照射后小鼠腹腔巨噬细胞阿黑皮素原 ( POMC)m RNA转录水平的时程变化。方法 :采用地高辛标记碱性磷酸酶显色原位杂交法。结果 :阳性细胞胞浆被染成蓝紫色 ,假照组有 POMC m RNA的表达 ,2 .0 Gy照射后不同时间点均未见有 POMC的表达。75m Gy照射后从 2～ 8h表达逐渐增加 ,8h达峰值 ,然后回降到假照水平。结论 :低剂量辐射可增强巨噬细胞 POMC转录 ,而高剂量则抑制其表达。

? Objective:The paper was to investigate the effect of irradiation (75 mGy,2.0 Gy) on the mRNA transcriptional level of POMC in peritoneal macrophages of mice.Methods:In situ hybridization (ISH) was used to examine the changes of POMC mRNA transcription level.Results:The positive cells were stained in blue purple color.The POMC mRNA transcription was increased from 2 h to 8 h after WBI with 0.075 Gy,returning to the control level at 16 h.No detectable POMC mRNA transcription was found at different time points after WBI with 2 Gy.Conclusion:Low dose irradiation could increase transcription level of POMC in peritoneal MΦ;whereas,high dose radiation could inhibit the POMC mRNA transcription level. 〔