摘要
以提取液吸光值为提取效果,通过分光光度计分析,确定70%乙醇为最佳浸提剂,提取液最大吸收波长λmax为440 nm。对蒲公英黄色素的提取进行了初步探索,优化蒲公英黄色素提取工艺,旨在开发新的天然色素资源,为工业化提取和精制蒲公英黄色素提供理论基础。通过超声处理,有机溶剂浸提法,选择无毒、无残留的乙醇作为浸提剂。通过回归正交旋转组合设计实验表明,料液比和浸提温度对蒲公英黄色素的提取影响较大。在优化条件下料液比1∶40(g/mL)、温度50℃、乙醇浓度70%下测得的最大OD值为0.816。
Seventy percent of ethanol and 440 nm λmax wavelength was deter mined to be suitable for extraction by spectrophotometer analysis.Extraction process of the yellow pigment from dandelion was optimized on the basis of the preli minary exploration.And it was designed to provide a theoretical basis for the industrialized extraction and refining of the pigment.Through organic solvent extraction,ethanol was deter mined as a soaker due to its better solvability for yellow pigment and non-toxicity and non-residue.Through regression orthogonal rotation design experiments showed that liquid ratio and extraction temperature on dandelion yellow pigment extracted a greater impact.The ratio of solid to liquid 1∶40(g/mL) with 70 % ethanol,ultrasound treatment 30 min,extraction time 3 h under the constant temperature of 50 ℃.Under the above conditions,the absorbance of the yellow pigment was 0.816.
出处
《食品研究与开发》
CAS
北大核心
2012年第10期98-102,共5页
Food Research and Development
基金
吉林省中医药管理局资助项目(ZY31)
白城市科技局资助项目(20070403)
关键词
蒲公英
黄色素
回归正交旋转组合设计
超声提取
优化
dandelion
yellow pigment
Regression orthogonal rotation
ultrasonic extraction
optimization