p-ERK1/2和c-Jun在人卵巢癌组织中的表达及意义

Expression of p-ERK1/2 and c-Jun in Human Ovarian Carcinoma and Its Significance

目的观察p-ERK1/2蛋白和c-Jun蛋白在卵巢肿瘤中的表达,探讨其与临床病理参数的关系。方法采用免疫组织化学MaxVisionTM法检测10例正常卵巢组织、20例卵巢良性肿瘤和40例卵巢癌组织的p-ERK1/2蛋白和c-Jun蛋白表达。结果卵巢癌中p-ERK1/2蛋白和c-Jun蛋白的表达明显高于正常卵巢组织及卵巢良性肿瘤(P<0.01),而卵巢良性肿瘤与正常卵巢组织中的p-ERK1/2和c-Jun表达差异无统计学意义(P>0.05)。p-ERK1/2蛋白和c-Jun蛋白在卵巢癌组织中表达与分化程度、年龄、临床分期和淋巴结转移均相关(均P<0.05);卵巢癌组织中p-ERK1/2蛋白的表达与c-Jun蛋白表达呈正相关(r=0.691,P<0.01)。结论 p-ERK1/2蛋白和c-Jun蛋白在卵巢癌组织中高表达,可为卵巢恶性肿瘤的诊断提供参考。

Objective To investigate the expression of phosphated extracellular-signal regula- ted kinase 1/2（p-ERK1/2）and c-Jun in human ovarian carcinoma,and to explore the relationship be- tween their expression and clinicopathologic parameters. Methods The expression of p-ERK1/2 and c-Jun was detected by MAXvisionTUplus immunohischemistry in 10 normal ovarian tissues,20 be- nign ovarian tumors and 40 ovarian carcinomas. Results Compared with ovarian carcinomas, the expression of p-ERK1/2 and c-Jun significantly decreased in normal ovarian tissues and benign ovarian tumors （P〈0.01）. However,there was no obvious difference in p-ERK1/2 and c-Jun ex- pression between normal ovarian tissues and benign ovarian tumors （P〉0.05）. In ovarian carci- nomas, the expression of p-ERK1/2 and c-Jun was correlated with tumor differentiation, age, clin- ical stage and lymph node metastasis （P〈0.05） ,and p-ERK1/2 expression was positively corre- lated with c-Jun expression （r=0. 691 ,P〈0.01）. Conclusion The p-ERK1/2 and c-Jun are high- ly expressed in ovarian carcinomas. The detection of p-ERK1/2 and c-Jun expression can provide a reference for the diagnosis of ovarian carcinomas.