RB基因异常改变在大肠癌发生、发展中的意义(英文)

Significance of Genetic Alterations of the RB Gene in Colorectal Tumorigenesis

目的 :深入了解RB基因过表达在大肠癌发生、发展中的意义。方法 :利用Northernblot,Dotblot在 1 6例大肠癌及 1 5例大肠腺瘤中测定RB基因的表达情况。运用核酸酶保护试验观察RB基因的点突变现象。结果 :发现在大多数大肠癌 ( 1 4/1 6 )及部分大肠腺瘤 ( 2 / 1 5 )中 ,同相应的正常大肠粘膜相比 ,存在着RB基因过表达现象。在 2例RB基因过表达的大肠癌标本中观察到RB基因发生了点突变。结论 :导致突变失活型Rb蛋白含量增高的RB基因异常改变与大肠癌发生。

Objective: To gain a better understanding of the mechanism of RB gene over expression in colorectal tumorigenesis. Method: The expression of the RB gene in 16 colorectal carcinomas and 15 adenomas was determined by Northern blot, Dot blot analysis. Applying RNase Protection assay, the point mutation in the RB gene was observed. Result: It was found that the most carcinomas and some adenomas have increased expression of RB gene compared with normal colon mucosa.The point mutation in the RB gene was found in two carcinomas with high level of the RB transcription.Conclusion: There is a close relationship between genetic alterations resulting in increased level of mutant RB proteins and the progression of colorectal carcinoma.