汤料中罂粟壳成分的检测

Determination of Alkaloid of Poppy Shell in Soup Materials in Chafing Dish

目的建立汤料中罂粟壳主要成分的检出及测定方法。方法用液相色谱定量检测Agilent SB-C18(4.6mm×150mm,5μm)色谱柱,以甲醇-0.02mol·L-1磷酸二氢钠溶液(5∶95)为流动相梯度洗脱,流速为1.0mL·min-1。结果吗啡、原阿片碱的线性范围分别为0.812～32.024μg·mL-1(r=0.9997)和1.702～68.103μg·mL-1(r=0.9998),平均回收率分别为96.0%和98.9%。结论该方法适用于汤料中罂粟壳主要成分的检出。

OBJECTIVE To establish a method for determination of Alkaloid of Poppy Shell in Soup Materials in Chafing Dish.METHODS Try paper primary screening with the morphine Diagnostic Strips,use HPLC for quantitative detection Agilent SB-C18（4.6mm×150mm,5μm） was used,Methyl alcohol-0.02mol·L-1 NaH2PO4（5∶95）as mobile phase for setting program,the flow rate was 1.0mL·min-1.RESULTS The linear ranges of morphine and protopine were in 4.007～160.3μg（r=0.9997） and 8.015～140.2μg（r=0.9998）,the average recoveries were 99.2% and 98.9%.CONCLUSION The method is convenient and effective,it can be used for determination of alkaloid of poppy shell in soup materials in chafing dish.