摘要
目的建立紫外分光光度法测定灯盏花素及其制剂中总黄酮含量的方法;方法采用紫外分光光度法,以野黄芩苷为测定指标,与三氯化铝反应,在353nm波长处测定;结果在线性范围内(4.16～20.80μg·mL-1)有良好的线性关系,r=0.9999,平均加样回收率为101.5%;结论所建立的含量测定方法简便可行,结果准确可靠,可用于灯盏花素制剂中总黄酮含量的测定。
OBJECTIVE To study method of determination of flavonoids content in Dengzhan huasu injection by UV spectrophotometry.METHODS The test index was scutellarin,the solution reacted with AlCl3 and detected at 353nm wavelength.RESULTS There was a good linear relationship between 4.16μg·mL-1 and 20.80μg·mL-1,the average recovery was 101.5%;CONCLUSION The established method is simple,feasible,and the result are correct and reliable,it can be used for determination of flavonoids contents in Dengzhan huasu injection.
出处
《海峡药学》
2012年第10期78-80,共3页
Strait Pharmaceutical Journal
关键词
灯盏乙素
紫外分光光度法
灯盏花素
总黄酮
Scutellarin
UV spectrophotometry
Dengzhan huasu injection
Flavonoids
