黄芩苷的抗炎机理

STUDY ON THE ANTI INFLAMMATORY MECHANISM OF BAICALIN

目的 :探讨黄芩苷抗炎作用机理。方法 :以大鼠腹腔白细胞为实验材料 ,分别采用高效液相色谱法(HPLC) ,放射免疫分析法 (RIA)和荧光分光光度法测定白细胞内白三烯 (LTs)、环一磷酸腺苷 (cAMP)和细胞内游离钙水平。结果 :黄芩苷可显著抑制白细胞内白三烯B4、白三烯C4的生物合成 ,其IC50 值分别为 0 4 8和 3 15μmol·L-1;黄芩苷还可显著抑制fMLP激发的白细胞内Ca2 +升高 ,并促进细胞内cAMP水平提高。结论 :黄芩苷显著影响白细胞的多种功能 ,提示此功能与其抗炎作用机理有关。

AIM: To investigate the effect of baicalin on leukocyte functions in attempt to elucide the mechanism of anti inflammatory action of baicalin. METHODS: Using rat peritoneal leukocyte as experimental material to determine the levels of leukotriene B 4, C 4(LTB 4, LTC 4), Ca 2+ and cAMP in the cells with HPLC, Fura 2/AM and RIA, respectively. RESULTS: Baicalin was shown to inhibit the biosynthesis of LTB 4 and LTC 4 with IC 50 value 0 48 and 3 15 μmol·L -1 , respectively. Baicalin at 1～100 μmol·L -1 showed no effect on cytoplasmic calcium concentration ([Ca 2+ ]i) of rat polymorphonuclear leukocytes (PMNL). However, baicalin at 10 and 100 μmol·L -1 inhibited the increase of Ca 2+ level induced by fMLP significantly by 29% and 40%, respectively. Baicalin at 1,10 and 100 μmol·L -1 showed a significant increase of the cAMP level in rat PMNL by 74%, 103% and 138%, respectively. CONCLUSION: The effects of baicalin on leukocyte function may be the mechanisms of its anti inflammatory action.