黄色素抑制血管平滑肌细胞增殖与3种蛋白激酶的关系

EFFECTS OF THREE KINDS OF PROTEIN KINASES ON SAFFLOWER YELLOW INHIBITION OF THE PROLIFERATION OF VASCULAR SMOOTH MUSCLE CELL

目的 :研究黄色素 (SY)对血管平滑肌细胞 (VSMC)增殖的作用及机制。方法 :以培养的家兔VSMC为材料 ,用细胞计数和32 P 参入法 ,观察SY对VSMC的增殖及 3种蛋白激酶活性。结果 :SY抑制VSMC的增殖 ,且呈剂量和时间依赖性 ,1 0mg·mL-1作用最强 ,抑制率为 5 4 6% ;SY抑制VSMC的DNA合成 ,1 0mg·mL-1作用 2d ,抑制率为 80 2 % ;在一定浓度范围内 ,随着SY浓度的升高 ,VSMC中 3种蛋白激酶 (TPK ,PKC及MAPK)活性下降。结论 :SY对VSMC的增殖有抑制作用 。

AIM: To study the effect of safflower yellow(SY) on the proliferation of vascular smooth muscle cell(VSMC) and the mechanisms.METHODS: The effect of SY on the proliferation of VSMC and the activities of three protein kinases (TPK,PKC,MAPK) in rabbit VSMC were studied by means of cell counting and 32 P incorporation.RESULTS: SY was shown to inhibit the proliferation of serum induced VSMC in dose and time dependent manner. SY at the dose of 1 0 mg·mL -1 significantly decreased the number of VSMC (the maxium inhibitory rate was 54 6%); The effect of SY on VSMC showed maxium inhibition of 52 0% for 4 days;the inhibitory rate decreased over 4 days. SY decreased the incorporation of 3H TdR in serum stimulated VSMC, the maxium inhibition was 80 2% at the dose of 1 0 mg·mL -1 for 2 days. As SY concentration increased, the activities of TPK,PKC and MAPK in serum stimulated VSMC decreased, SY inhibited the activities of TPK,PKC and MAPK in VSMC and reach maxium at the dose of 1 0 mg·mL -1 for 3 days(97 8%, 92 9% and 82 3% respectively). CONCLUSION: SY inhibited the proliferation of VSMC, the inhibitory effect of SY on VSMC proliferation might be due to inhibition of TPK, PKC and MAPK.