胆固醇酯转运蛋白(CETP)―TaqIB基因多态性与散发性阿尔茨海默病相关性研究

Relationship Between Cholesteryl Ester Transfer Protein(CETP)-TaqIB Gene Polymorphism and Sporadic Alzheimer's Disease

目的:研究散发性阿尔茨海默病(sporadic Alzheimer’disease,SAD)患者和正常老年人之间血清脂质水平、认知功能的差异;探讨胆固醇酯转运蛋白(CETP)-TaqIB基因多态性与SAD的关系及其对认知功能的影响。方法:对100例散发性阿尔茨海默病患者和102例健康对照者进行简易精神状态量表(MMSE)、Hachinski缺血指数量表(HIS)评分,其中49例轻-中度AD患者、61例健康对照者接受韦氏记忆量表(WMS)及威斯康辛卡片分类测验(WCST)的测量;采用氯仿法抽提外周血白细胞基因组DNA、多聚酶链式反应和限制性片段长度多态性法(PCR-RFLP)测定CETP-TaqIB基因型。结果:AD组血清胆固醇水平高于对照组(P<0.05);AD组中CETP-TaqIB三种基因型频率分别为B1B1 35%、B1B2 48%、B2B2 17%,CETP-TaqIB基因型和等位基因频率分布在阿尔茨海默病组(AD)与对照组之间无显著性差异(P>0.05);研究总人群中,CETP-TaqIB三种基因型的WMS各分测验成绩差异无显著性(P>0.05),而B2等位基因携带者长时记忆评分高于B1B1纯合子(P<0.05);B1B1、B1B2和B2B2基因型的WCST中完成分类数呈下降趋势,持续性反应呈上升趋势,但差异无统计学意义(P>0.05)。结论:CETP-TaqIB基因多态性与阿尔茨海默病发病无关,CETP-TaqIB基因多态性可能与老年人长时记忆功能差异有关。

Objective： To explore the difference in the cognition function and the level of blood profile between Alzheimer＇s disease patients and healthly aged controls.To investigate the association beween the cholesteryl ester transfer protein（CETP） TaqIB polymorphism and Alzheimer＇s disease,and the patient＇s cognition function.Methods： 100 AD patients and 102 controls were assessed with mini-mental state examination（MMSE） and Hachinski ischemic score（HIS）;at the same time,49 mild-to-modetate patients with AD and 61 healthy controls were assessed with Wechsler Memory Scale（WMS） and Wisconsin card sorting test（WCST）.Polymerase chain reaction-restricted fragments length polymorphism（PCR-RFLP） was used to determine CETP-TaqIB gene polymorphism.Results： The CETP-TaqIB genotype frequencies of AD patients were B1B1 35%、B1B2 48% and B2B2 17%.There was no significant association in CETP-TaqIB genotype and allele distribution between AD patients and the healthy controls（P0.05）.In the study population,there was no significant difference in the score of WMS among CETP-TaqIB B1B1 genotype,B1B2 genotype and B2B2 genotype,but B2 allel gene had higher score than B1B1 gentype（P0.05）;Complete classification number was gradually reduced among the three genotypes.Sustained response was gradually increased,but there were no obvious differences.Conclusion： There was no obvious correlation between CETP-TaqIB gene polymorphism and Alzheimer＇s disease,CETP-TaqIB polymorphism might be associated with long-term memory in elder people.