纳洛酮在细胞信号传递系统中对蛋脑啡肽的拮抗作用

EFFECTS OF NALOXONE AGAINST METHIONINE-ENKE-PHALIN ON THE SIGNAL TRANSMISSION SYSTEM IN MOUSE NS-1 CELLS

目的 :探讨蛋脑啡肽 (MENK)在细胞信号传递系统的作用及其方式。方法 :用蛋脑啡肽及纳洛酮处理小鼠的骨髓瘤细胞 (NS-1 ) ,之后测定蛋白激酶 A(PKA)和蛋白激酶 C(PKC)活性。结果 :表明蛋脑啡肽可以升高 NS-1细胞胞浆 PKA活性 ,却降低胞浆 PKC活性 ,而且这 2种作用均能被不同浓度的纳洛酮所拮抗。结论 :蛋脑啡肽通过传统的阿片受体机理参与了 2条 (c AMP-PKA途径及 DG-PKC途径 )细胞外信号传递系统的信号传递。

Objective: Mouse NS 1 cells were treated with methionine enkephalin(MENK) and different concentrations of naloxone in order to study the biological effects and mechanism of methionine enkephalin on the signal transmission system. Method: Activities of proteir kinase A(PAK) and protein kinase C (PKC) were measured. Results: The results showed that MENK could enhance the activity of PKA and decrease the cativity of PKC in the cytoplasm of NS 1 cells that were antagonized by nalosone. Conclusion: Coupled with all the findings, it might be inferred that two signal transmission systems(cAMP PKA route and DG PKC route) were involved in the effects of MENK by bindind the traditional opioid receptors and, therefore, resulted in different biological effects.