TACC3 mRNA及蛋白在乳腺癌中的表达及其临床意义

mRNA and Protein Expressions of Transforming Acidic Coiled-Coil Protein3 and its Clinical Significance in Breast Cancer

目的:探讨转录相关酸性卷曲蛋白3(transforming acidic coiled-coil proteins,TACC3)mRNA和蛋白在乳腺癌中的表达及其临床意义。方法:收集手术切除乳腺癌及癌旁正常组织标本各85例,进行免疫组织化学染色、RT-PCR、Western blot的检测。结果:乳腺癌组织中的TACC3 mRNA和蛋白的表达水平高于癌旁组织(P<0.05),且TACC3的表达与乳腺癌的分化程度、肿瘤直径、淋巴结的转移、P53表达相关,而与患者的年龄、是否绝经、ER、PR、Ki-67、HER-2等无明显相关。结论:TACC3在乳腺癌中的表达程度与临床病理特征有关,在乳腺癌发生发展过程中可能具有一定的作用。

Objective： This work aims to investigate the mRNA and protein expressions of transforming acidic coiled-cot protein3 （TACC3） as well as its clinical significance in breast cancer. Methods： A total of 85 breast cancer tissues and paraneoplastic breast tissues were collected as samples. The mRNA and protein expressions of TACC3 in these tissue samples were determined using reverse transcription-polymerase chain reaction, western blot analysis, and immunohistochemical staining. Results： The mRNA and protein expressions of TACC3 were significantly higher in the breast cancer tissues than in the paraneoplastic breast tissues （P〈0.05）. The TACC3 expressions were significantly correlated with the degrees of differentiation, tumor sizes, nodal metastasis, and tumor protein 53, but not with the ages of patients, menstrual status, and the expressions of estrogen receptor-positive, progesterone receptor-positive, human epidermal growth factor receptor-2, and Ki-67. Conclusion： TACC3 was overexpressed in breast cancer. This result is relevant to the clinicopathological features of the disease. Therefore, TACC3 expression may play a significant role in the onset and progression of breast cancer.