乌药总生物碱提取工艺的优选及镇痛作用的研究

Optimization of extraction technology and analgesic effect of total alkaloids from Linderae Radix

目的优选乌药总生物碱(TALR)的提取工艺并研究其镇痛作用。方法采取乙醇回流法提取,考察乙醇浓度、乙醇用量、提取时间、提取次数影响,进行L9(34)正交试验设计,以乌药总生物碱的紫外吸收峰最大吸光度A值为指标进行优选。镇痛实验取最佳提取方法所得TALR的不同浓度水溶液ig给予小鼠后,采用醋酸小鼠扭体法研究TALR的镇痛作用。结果优选工艺为:加入14倍量95%乙醇提取1h,酸溶碱沉得TALR,因其A值远高于其他工艺,故判断此法所得TALR质量分数相对最高。药理实验表明TALR可显著减少小鼠扭体次数。结论优选工艺简单可行,质量分数较高;TALR具有较强的镇痛作用。

Objective To optimize the extraction technology of total alkaloids from Lindera Radix（TALR） and to study the analgesic effect.Methods The extraction technology of TALR was optimized by orthogonal experiment with concentration of ethanol,amount of ethanol,extraction time,and extraction times as factors,and with the maximum absorbance of ultraviolet absorption peak（A） of TALR as index.In analgesic experiments,different concentration of TALR aqueous solution extracted by the optimized method was ig administered to mice,and the analgesic effect of TALR was observed by acetic acid writhing method.Results The optimum technology was 1 h extraction with 14 times amount of 95% alcohol and TALR was got after acid-dissolution and alkali-precipitation.The A value of optimum technology outclassed others,so the content of TALR was higher.Pharmacologic experiments indicated TALR could obviously decrease mouse writhing times.Conclusion The optimum technology is easy to be operated and extracted TALR has higher content.TALR has obvious analgesic effect.