摘要
目的:探讨血浆miR-143和miR-145水平与冠脉粥样硬化严重程度的关系。方法:112例患者按冠脉造影结果分为冠脉病变组、非显著冠脉病变组和对照组,用Gensini评分评估病变严重程度。用实时定量RT-PCR检测血浆中miR-143和miR-145的浓度,并评估其与冠脉粥样硬化严重程度的关系。结果:与非显著冠脉病变组和对照组相比,冠脉病变组患者血浆miR-143和miR-145水平显著降低。miR-143和miR-145是冠脉粥样硬化严重程度的独立危险因素(miR-143,r=-0.440,P=0.001;miR-145,r=-0.459,P=0.015)。miR-143和miR-145的ROC曲线下面积分别为0.884和0.909。结论:miR-143和miR-145可作为评估冠脉粥样硬化程度的生物标志物,并具有较高的特异性和敏感性。
Objective,- To investigate relationship between miK-i4~, mux-~~o anu t,le be y u, coronary atherosclerosis. Methods: The plasma levels of miR-143 and miR-145 were examined by real-time RT-PCR in 112 subjects, which were divided into coronary artery disease group (CAD)group, non-signifi- cant CAD group and healthy group according to angiography results, miR-143 and miR-145 were measured by RT-PCR, and the relationship between them severity o[ coronary atherosclerosis was also analyzed. Re- suits. Significant decreases were detected in plasma levels of miR-143 and miR-145 in patients with CAD compared with non-significant CAD or healthy subiects. In addition, miR-143 and miR-145 were identified as independent factors associated with the severity of atherosclerosis (miR-143, r=- 0. 440, P = 0. 001; miR-145,r= --0. 459, P = 0. 015). Furthermore, receiver operating characteristic curves analysis showed miR-143 and miR-145 were diagnostic predictors in CAD patients, with an area under curve of 0. 884 and 0. 909, respectively. Conclusions: miR143 and miR145 may function as useful biomarkers with high sensitivity and specificity in the diagnosis of initiation and development of coronary atherosclerosis.
出处
《海南医学院学报》
CAS
2012年第11期1526-1528,1536,共4页
Journal of Hainan Medical University
基金
国家自然基金青年基金(81100130)~~