血浆miR-143、miR-145与冠脉粥样硬化程度的关系

Relationship between miR-143,miR-145 levels and severity of coronary atherosclerosis

目的:探讨血浆miR-143和miR-145水平与冠脉粥样硬化严重程度的关系。方法:112例患者按冠脉造影结果分为冠脉病变组、非显著冠脉病变组和对照组,用Gensini评分评估病变严重程度。用实时定量RT-PCR检测血浆中miR-143和miR-145的浓度,并评估其与冠脉粥样硬化严重程度的关系。结果:与非显著冠脉病变组和对照组相比,冠脉病变组患者血浆miR-143和miR-145水平显著降低。miR-143和miR-145是冠脉粥样硬化严重程度的独立危险因素(miR-143,r=-0.440,P=0.001;miR-145,r=-0.459,P=0.015)。miR-143和miR-145的ROC曲线下面积分别为0.884和0.909。结论:miR-143和miR-145可作为评估冠脉粥样硬化程度的生物标志物,并具有较高的特异性和敏感性。

Objective,- To investigate relationship between miK-i4~, mux-~~o anu t,le be y u, coronary atherosclerosis. Methods： The plasma levels of miR-143 and miR-145 were examined by real-time RT-PCR in 112 subjects, which were divided into coronary artery disease group （CAD）group, non-signifi- cant CAD group and healthy group according to angiography results, miR-143 and miR-145 were measured by RT-PCR, and the relationship between them severity o[ coronary atherosclerosis was also analyzed. Re- suits. Significant decreases were detected in plasma levels of miR-143 and miR-145 in patients with CAD compared with non-significant CAD or healthy subiects. In addition, miR-143 and miR-145 were identified as independent factors associated with the severity of atherosclerosis （miR-143, r=- 0. 440, P = 0. 001; miR-145,r= --0. 459, P = 0. 015）. Furthermore, receiver operating characteristic curves analysis showed miR-143 and miR-145 were diagnostic predictors in CAD patients, with an area under curve of 0. 884 and 0. 909, respectively. Conclusions： miR143 and miR145 may function as useful biomarkers with high sensitivity and specificity in the diagnosis of initiation and development of coronary atherosclerosis.