大鼠口服裸花紫珠后木犀草素的体内药动学研究

Pharmacokinetic study of luteolin from Callicarpanudiflora in rats

目的:建立木犀草素血药浓度的高效液相色谱测定方法,并探讨大鼠口服裸花紫珠提取物后木犀草素的体内药代动力学行为。方法:采用phenomenex C18(5u,250mm×4.6mm)色谱柱,流动相为甲醇-水(52∶48),流速1.0 mL·min-1,柱温:30℃,检测波长350nm。大鼠灌胃裸花紫珠提取物20g·kg-1后,与不同时间点股静脉取血,用HPLC法测定其血药浓度,绘制药-时曲线。结果:大鼠血浆中,木犀草素在0.005～0.10μg范围内,回归方程为Y=29063X-1933.3,r=0.9989,呈良好的线性关系。大鼠灌胃裸花紫珠提取物后,木犀草素的药-时曲线符合二室模型。结论:裸花紫珠提取物中木犀草素在大鼠体内吸收迅速。该方法专属性强,灵敏度高,可用于木犀草素在大鼠体内的药动学研究。

Objective. To establish an HPLC method for the determination of luteolin in rat plasma and to study the pharmacokinetics of luteolin in rats. Methods. An phenomenex C18（5u, 250 mm×4. 6 mm） column was adopted, the mobilephase was a mixture of methyl alcohol and water（52 ＂ 48） at the flow rate of 1.0 mL/min and the UV detector was operated at 350 nm. After oral administrat-ion of Calli- carpanudiflora （20 g/kg）, the luteolin concentration in rat plasma was detected by HPLC. The pharmaeo- kinetic parameters were calculated by WinNonlon 5.2 program. Results. In rat plasma, a good linearity of luteolin was found in the range of 0. 005-0. 10 #g （r= 0. 9989）. The plasma luteolinconcentration-time curve could be evaluated by the two compartment modes. Conclusions. Luteolin can be absorbed quickly in rats. It is specific and sensitive, and can be used in the pharmacokinetic study of luteolin of rat in vivo.