摘要
目的建立复方桔梗麻黄碱糖浆(Ⅱ)的质量标准。方法采用薄层色谱法进行定性鉴别,以反相高效液相色谱法测定盐酸麻黄碱的含量。采用C18柱,流动相为乙腈-0.1%磷酸溶液(含0.1%三乙胺)(5∶95),检测波长为210nm。结果薄层色谱分离度较好,盐酸麻黄碱的线性范围为40.94~1 637.6 ng(r=0.999 9,n=5),平均回收率为100.8%(RSD=1.1%,n=9)。结论该法简便、准确、专属性强,可有效地控制复方桔梗麻黄碱糖浆(Ⅱ)的质量。
Objective To establish a method for the quality control of Compound Balloonflower and Ephedrine Syrup( Ⅱ ). Methods TLC was used to qualitatively identify and RP - HPLC was used to determine ephedrine hydrochloride in the Compound Balloonflower and Ephedrine Syrup. C18 column was adopted, the mobile phase was acetonitrile -0.1% phosphoric acid ( containing 0.1% triethylamine) ( 5 : 95 ) , and the detection wavelength was 210 nm. Results The herbs could be identified by TLC. The linear range of ephedrine hydrochloride was from 40.94 N 1 637.6 ng( r = 0. 999 9, n = 5 ) and the average recovery was 100.8% (RSD = 1.1%, n = 9). Conclusion The method was simple, accurate and specific, and it can be applied to the quality control of the Compound Balloonflower and Ephedrine Syrup.
出处
《齐鲁药事》
2012年第10期574-576,共3页
qilu pharmaceutical affairs