摘要
研究枸骨叶中熊果酸的最佳提取工艺条件。在单因素试验的基础上,采用正交试验设计法,选择乙醇浓度（A）、固液比（B）、提取时间（C）为因素,每因素各取三水平,按L_9（3~4）正交表进行实验。结果表明：影响提取效果各因素为乙醇浓度〉提取时间〉固液比。其最优提取工艺条件为用8.5倍量φ=95%乙醇,于80℃加热回流提取2次,第一次2h,第二次1.5h。
To study the best extraction process of ursolic acid in leaves of Ilex cornuta.Based on the single-factor test,experiment was carried out according to L9（34） orthogonal table by orthogonal experimental design method,selecting the ethanol concentration（A）,solid-liquid ratio（B）,extraction time（C） as factors,three levels for each factor.The results showed that the factors affecting the extraction were ethanol concentration extraction time solid-liquid ratio.The optimum extraction conditions were that heating reflux extraction were done for two times using 8.5 times the amount of φ = 95% ethanol at 80 ℃,2 h for the first,1.5 h for the second.
出处
《井冈山大学学报（自然科学版）》
2012年第6期21-23,共3页
Journal of Jinggangshan University (Natural Science)
基金
国家"863"高技术研究发展计划项目[2002AA2Z3217]
关键词
枸骨叶
熊果酸
提取工艺
正交实验
Ilex cornuta leaves
ursolic acid
extraction process
orthogonal experiment
