摘要
利用扩增片段长度多态性(AFLP)技术分析了田头菇属的茶树菇(Agrocybe aegerita)及杨柳田头菇(Agrocybe.salicacola)基因组DNA的多态性,使用11对引物组合建立了18个供试菌株的指纹图谱。结果表明:通过聚类分析,18个供试菌株聚为两大类,彼此关系得到很好的分辨。AFLP技术可用于茶树菇和杨柳田头菇2个形态上非常相似物种的DNA分子指纹图谱构建。
AFLP technique was used to analyze the genomic DNA polymorphism of Agrocybe aegerita and A.salicacola collected from Yunnan.The DNA fingerprint of the eighteen strains in Agrocybe was generated by eleven primer combinations.Eighteen strains of Agrocybe were clustered into two big groups,which phylogenic relationship was distinguished clearly.The results indicated that AFLP technique could be used to construct the DNA molecular fingerprint of A.aegerita and A.salicacola,both of which were very similar in morphology.
出处
《北方园艺》
CAS
北大核心
2012年第21期85-88,共4页
Northern Horticulture
基金
云南省自然科学基金资助项目(2011FZ214)
国家自然科学基金资助项目(31101591
31160160)
国家食用菌产业技术体系资助项目(CARS-24)
关键词
茶树菇
杨柳田头菇
DNA指纹图谱
聚类分析
Agrocybe salicacola
Agrocybe aegerita
DNA fingerprint
cluster analysis
