摘要
以兴安杜鹃的叶片为外植体,研究其离体培养技术。结果表明:最佳愈伤组织诱导培养基为1/4Anderson+2,4-D 1mg/L+TDZ 0.1mg/L,最佳芽诱导培养基为1/4Anderson+IBA 0.1mg/L+TDZ 1mg/L+2-IP 0.5mg/L,最佳生根培养基为1/4Anderson+IBA 2mg/L或1/4Anderson+NAA 0.1mg/L。
Taking the leaves as material,the rapid propagation culture in vitro of Rhododendron dauricum were studied.The results showed that the best callus induction medium was 1/4Anderson＋2,4-D 1 mg/L＋TDZ 0.1 mg/L;the best bud induction medium was 1/4Anderson＋IBA 0.1 mg/L＋TDZ 1 mg/L＋2-IP 0.5 mg/L;the best rooting culture medium was 1/4Anderson＋IBA 2 mg/L or the medium of 1/4Anderson＋NAA 0.1 mg/L.
出处
《北方园艺》
CAS
北大核心
2012年第21期98-100,共3页
Northern Horticulture
基金
吉林省教育厅"十二五"科学技术研究资助项目(吉教科合字(2011)第279号)
关键词
兴安杜鹃
叶片
培养基
离体培养
Rhododendron dauricum L
leaf
medium
culture in vitro
