情志刺激致大鼠肝郁脾虚证模型的研究

Emotional stimulation-induced rat model of liver stagnation spleen deficiency

目的:研究慢性束缚加饮食失节孤养模拟肝郁脾虚病因,创立复合病因大鼠造模方法。方法:将大鼠束缚于网笼中,令其肢体不能自由活动,每日束缚6h,孤养,每周不定时禁食2次,连续3周;另设疏肝健脾方逍遥丸、疏肝泻火方龙胆泻肝丸作对照,正常对照鼠合养,自由活动、饮水,观察一般状况、精神行为、血液免疫器官及脑组织单胺类神经递质变化评价肝郁脾虚证。结果:模型对照组大鼠体质量增长、糖水消耗量、水平和垂直运动、血红蛋白、血小板减少、胸腺和脾指数、5-羟色胺(5-HT)和多巴胺(DA)显著低于空白对照组,水中静止时间显著高于空白对照组(P<0.05,P<0.01);逍遥丸组与模型对照组比较,其差异也有统计学意义(P<0.05,P<0.01)。结论:慢性束缚加饮食失节孤养的方法成功创立符合中医证候特征的肝郁脾虚模型。

Objective： Chronic restraint plus abnormal diet,solitary was taken to simulating etiology of liver stagnation spleen deficiency,which created compound etiology causing model.Method： Rats were constrained in cages for 6h everyday,solitary and fasting twice every week,and this stage lasted 3w.Xiaoyao Pills and Longdan Xiegan Pills were taken as control drugs.Activity,drinking water,common condition,mental action,monamine neurotransmitter of blood immunity organs and brain were observed as indexes to evaluate liver stagnation spleen deficiency syndrome.Results： Compared with normal group,weight increase,glucose water consumption,horizontal and vertical motion were lower,hemoglobin and platelet decrease,thymus and spleen index,5-HT and DA content were significantly lower,while quiescent time in water was significantly higher（P0.05,P0.01）.Compared with model group,Xiaoyao Pill group was on the controary（P0.05,P0.01）.Conclusion： Chronic restraint plus adnormal diet,solitary ways successfully created was in line with traditional Chinese medicine syndromes of liver stagnation spleen deficiency model.