植物精油对鱼藤酮和辣椒碱在斜纹夜蛾离体培养细胞系SL-1中活性的影响

Effects of Essential Oil on the Cytotoxicity of Rotenone and Capsaicin Against Spodoptera litura Cultured Cell Line SL-1 in Vitro

应用流式细胞术研究了24种常用植物精油对斜纹夜蛾Spodoptera litura离体培养细胞系(SL-1)细胞膜通透性的影响,采用MTT法研究精油对2种植物源农药鱼藤酮和辣椒碱的细胞活性的影响.试验结果表明:与对照相比,50μg·mL-1芸香油处理SL-1细胞24 h后,细胞内荧光染料碘化丙啶(PI)的荧光强度增加了53.04%,其次是松节油和当归油,细胞内PI荧光强度分别增加了34.23%和33.67%;100μg·mL-1广藿香油、松节油、芸香油、当归油、香茅油、生姜油处理SL-1细胞后,细胞内PI荧光强度增加率超过40%.鱼藤酮和辣椒碱对SL-1细胞的抑制中浓度(IC50)分别为34.97和35.92μg·mL-1,而与100μg·mL-1芸香油联用时IC50分别为12.69和13.26μg·mL-1,与100μg·mL-1松节油联用的IC50分别为14.56和11.392μg·mL-1,均小于鱼藤酮和辣椒碱单剂用量,芸香油、松节油能够显著提高鱼藤酮和辣椒碱对SL-1细胞的细胞活性.

Flow cytometry （FCM） was used to analysis the destruction of essential oils on membrane in- tegrity of Spodoptera litura cultured cell line SL-1. The cytotoxicity of rotenone and capsaicin alone or in combination with essential oils against SL-1 cells were studied by MTY method. Flow cytometric analysis showed that the intra-cellular fluorescence intensity of accumulation in the membrane impermeable nucle- ic acid stain propidium iodide （PI） was promoted by 53.04% after 24 h treatment with 50 μg . mL-1 of rue oil , followed by 34. 23% and 33.67% after treatment with angelica oil and turpentine oil, respec- tively. After being treated with 100μg. mL- 1 of patchouli oil, turpentine oil, rue oil, angelica oil, cit- ronella oil, and ginger oil, the increase rates of the accumulation of PI were all higher than 40%. The values of median inhibitory concentration （ IC50 ） for rotenone and eapsaicin against SL-1 cells for 24 h were 34. 97 and 35.92 μg . mL-1 , respectively. When combined with 100 μg . mL-1 of rue oil, the values of IC50 were decreased to 12. 69 and 13.26 μg . mL-1, and the other values were 14. 56 and 11. 392 μg.mL-1 when combined with 100 μg. mL-1 of turpentine oil, which were all lower than thosetreated with rotenone and capsaicin only. All these results indicated that rue oil and turpentine oil could obviously enhance the cytotoxicity with rotenone and capsaicin.