摘要
为研究山梨酸对小鼠骨骼肌成肌细胞(C2C12)增殖、IGF-1分泌、GH/IGF系统和糖脂代谢相关基因表达的影响,试验通过培养基中添加不同浓度的山梨酸(0、0.1、1.0、10.0和100.0μmol/L)处理C2C12细胞,采用MTT法检测细胞增殖,RIA法检测细胞IGF-1分泌量,qPCR法检测相关基因mRNA的表达水平.结果表明:1)10.0μmol/L山梨酸显著促进体积分数为10%胎牛血清(1和2 d)和无血清(4和5 d)培养的C2C12细胞增殖(P<0.05);2)0.1、1.0和100.0μmol/L山梨酸均显著抑制无血清培养的C2C12细胞IGF-1的分泌(P<0.05);3)1.0μmol/L山梨酸显著上调无血清培养的C2C12细胞IGF-1R、GHR和CPT1b的mRNA表达(P<0.05),对IGFBP3(P=0.09)、PDK4(P=0.10)和PGC1α(P=0.10)的mRNA表达量也有提高趋势.提示山梨酸可促进C2C12细胞的增殖和GH/IGF系统及糖脂代谢相关基因mRNA表达,但对IGF-1的分泌具有抑制作用.
The purpose of this experiment was to explore the effects of sorbic acid （SA） on proliferation, IGF-1 secretion, GH/IGF system and glucose and lipid metabolism-related genes mRNA expression in C2C12 cells. C2C12 cells were exposed to different doses of SA（0, 0. 1, 1.0, 10. 0 and 100. 0 μmol/L） to measure cell proliferation by MTF assay, IGF-1 secretion by RIA assay and transcript levels by qPCR as- say. Results indicated that ： 1 ） SA （ 10. 0 μmol/L） significantly promoted （ P 〈 0. 05 ） proliferation of cell cultured with 10% fetal bovine serum（ 1 and 2 d） or without serum（4 and 5 d）. 2） IGF-1 secretion was significantly suppressed （ P 〈 0. 05 ） by 0. 1, 1.0 or 100. 0 μmol/L SA in serum-free cultured C2C12 cells. 3） Expressions of IGF-1 R, GHR and CPTlb mRNA were significantly up-regulated in serum-free cultured C2C12 cells treated with 1.0μmol/L SA. The increasing trend of IGFBP3 （P = 0. 09） , PDK4 （P =0. 10） and PGC1α（P =0. 10） transcript levels were also observed in serum-free cultured C2C12 cells by 1.0 μmol/L SA treatment. This suggested that SA could increase proliferation and GH/IGF sys- tem and glucose and lipid metabolism-related genes mRNA expression but depress IGF-1 secretion in C2C12 ceils.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2012年第4期529-534,共6页
Journal of South China Agricultural University
基金
国家重点基础研究发展计划(2012CB124701)
企业横向课题<猪用抗生素替代品--新型高效有机酸制剂的研发>
