接种菌根菌短枝木麻黄对低温胁迫的响应特征

Response Characteristics of Casuarina equisetifolia Inoculated with Mycorrhizal Fungi under Low Temperature Stress

通过对接种不同菌根菌短枝木麻黄幼枝进行系列低温胁迫处理,分析抗寒性相关生理指标与接种菌根菌短枝木麻黄抗寒性关系,探讨短枝木麻黄接种菌根菌对低温胁迫的响应特征,为科学评价和改良短枝木麻黄的抗寒能力提供依据。结果表明:(1)接种菌根菌有助于短枝木麻黄形成菌根,可显著促进其苗期生长。(2)随着胁迫温度的降低,接种菌根菌短枝木麻黄嫩枝SOD、POD、CAT活性均呈先上升后下降的趋势,MDA含量逐渐增加,且细胞膜透性均升高。(3)与相同温度处理下未接种对照相比,7个接种处理短枝木麻黄的嫩枝SOD、POD、CAT活性显著提高,并在0～4℃达到最高值,其MDA含量和细胞膜透性显著降低,而且各菌株处理的升降幅度存在显著差异。研究发现,在一定低温胁迫下,接种菌根菌可以显著提高短枝木麻黄嫩枝主要保护酶活性,有效降低其细胞膜透性和MDA含量,从而提高自身抗寒能力;接种内生菌根菌苏格兰球囊霉和外生菌根菌多根硬皮马勃对短枝木麻黄耐寒性的促进效果最佳。

In this experiment,Casuarina equisetifolia was inoculated with different mycorrhizal fungi shoots under low temperature stress.The relationship between cold resistant physiological indicators and inoculation of mycorrhizal fungi of C.equisetifolia was analyzed in order to investigate the response characteristics of C.equisetifolia inoculated with mycorrhizal fungi under low temperature stress,and provide the basis for scientific evaluation and improvement of cold tolerance of C.equisetifolia.The results show that：（1）Inoculated with mycorrhizal fungi could significantly promote the growth of C.equisetifolia seedlings.（2）With the reduction of the stress temperature,SOD,POD,CAT activities of inoculated with mycorrhizal fungi of C.equisetifolia twigs were all increased and then decreased trend.MDA content and cell membrane permeability increased with decreasing temperature.（3）Compared with the unvaccinated control at same temperature the activities of SOD,POD,CAT of seven inoculated twigs of C.equisetifolia were significantly increased （maximum when 0～4℃） and a significant reduction in MDA content and cell membrane permeability,moreover there were significant difference between treaments.Study found that inoculated under appropriate low temperature stress with mycorrhizal fungi could significantly improve the protection of enzyme activity,reduce cell membrane permeability and MDA content of C.equisetifolia twigs to improve their resistance to cold.Inoculated AMF Glomus caledonium Nicoison ＆ Ceelemann and ECMF Scleroderma polyrhizum Pers had the best promoting effect on C.equisetifolia cold resistance.