雷公藤组培产物的杀虫杀菌活性研究

Insecticidal and Antifungal Activity of Tripterygium wilfordii Cultures

采用室内生测法研究了雷公藤愈伤组织、悬浮细胞、不定根等组培产物对小菜蛾毒杀、生长发育影响以及不定根提取物对番茄灰霉病等植物病原真菌菌丝生长抑制作用,以明确雷公藤组培产物的生物活性及其应用前景。结果表明,不同雷公藤组培产物对小菜蛾3龄幼虫都具有明显的毒杀作用,悬浮细胞以及不定根的LC50均超过了根皮粉的效果,其中的不定根提取物对小菜蛾3龄幼虫毒杀作用的LC50为根皮粉的1.95倍;不同组培产物提取物对小菜蛾幼虫的生长均有明显的抑制作用,其中不定根提取物处理后每天的小菜蛾体重显著下降,72h后70%左右已经死亡,存活的试虫体重比试验前下降了18.33%;雷公藤不定根提取物对供试的11种植物病原真菌菌丝生长均有一定的抑制作用,有5种的抑制率超过60%,并对番茄灰霉病菌的抑制作用最强,其EC50为10.074mg/mL,且不定根的抑制效果均超过了根皮粉的抑制效果。

The cultures from Tripterygium wilfordii were bioassayed with Plutella xylostella insecticidal activity and to reveal the antifungal activity of T.wilfordii adventitious roots.The results showed that all cultures had obvious inhibition and toxicity effect on P.xylostella.The LC50 of suspended cells and adventitious roots are all more than that of root bark,and the LC50 of adventitious roots was 1.95 times of root bark.The extract of different cultures significantly inhibited the growth and development of P.xylostella.After the treatment of the adventitious roots extraction,P.xylostella showed dramatically reduction on body weight,about 70% P.xylostella died after 72 hours and the weight of the survivals decreased by 18.33% after the test.The adventitious roots inhibited the mycelial growth of 11 species of plant-pathogenic fungal in vitro.The inhibition rate of adventitious roots was exceeded 60% of 5 plant-pathogenic fungal species in vitro.It markedly inhibited the mycelial growth of Botrytis cinerea.The EC50 value against B.cinerea was 10.074 mg/mL.The inhibitory effect of adventitious roots was exceeded the root bark in all tests.