Maspin蛋白在鼻咽癌放射敏感CNE2细胞株中的表达分析

Expression of Maspin in radiosensitive nasopharyngeal carcinoma CNE2 cell line

目的探讨放射敏感鼻咽癌CNE2细胞株在放射条件下丝氨酸蛋白酶抑制剂Maspin蛋白的反应性变化,分析鼻咽癌放射作用的分子机制。方法采用MTT比色法检测放射线对鼻咽癌CNE2细胞株生长的影响,胎盼蓝染色法检测CNE2细胞增殖,流式细胞术检测CNE2细胞放射后的凋亡及周期改变,Western blotting分析Maspin蛋白的表达。结果鼻咽癌CNE2细胞株放射后,细胞周期重新分布,出现较多的G2/M期细胞以及较少的G0/G1期细胞;增殖受到抑制、细胞凋亡明显、Maspin蛋白表达上调,且其上调与细胞凋亡率增高呈现一致性改变,也与细胞周期分布改变相关。结论鼻咽癌CNE2细胞对放射线敏感、容易被诱发凋亡;细胞周期参与了放射诱导的CNE2放射反应调节;放射提高了CNE2细胞的Maspin蛋白表达,提示该蛋白参与了CNE2细胞的放射反应;Maspin蛋白表达提高涉及CNE2细胞周期和凋亡改变,是一个值得深入研究的鼻咽癌放射敏感相关分子。

Objective To study the responsive changes of Maspin protein in radiosensitive nasopharyngeal carcinoma （NPC） cell line CNE2 when irradiated, for analyzing the molecular mechanism of radiotherapy for rqPc. Methods MTT method was utilized to test the effect of radioactive rays on the growth of CNE2 cell line, flow eytometry to detect the cell apoptosis and cell cycles of CNE2 cell line, and Western Blot to analyze the expression of Maspin protein. Results After being irradiated, the cell cycles of CNE2 cell line were redistributed with increase of G2/M stage cells and decrease of G0/G1 stage cells, cell multiplication was inhibited, apoptosis was enhanced, and the expression of Maspin protein was up-regulated. The up-regulation of Maspin expression was consistent with the enhancement of cell apoptosis, and was also related to cell cycle redistribution. Conclusion CNE2 cells are radiosensitive and apoptosis is apt to be induced under irradiated situation. Cell cycles participate in the responsive regulation of CNE2 cells induced by radiation. The expression of Maspin protein can be up-regulated by radiation, which suggests that Maspin may participate in the responsive regulation of CNE2 cells. The expression of Maspin is involved in cell cycle and apoptosis, and therefore Maspin is a radiosensitive molecular worthy of further study in NPC.