中药海尔福口服液对砷中毒小鼠的解毒效果研究

Detoxification effects of traditional Chinese medicine of Haier Fu oral liquid on mice with arsenic poisoning

目的探讨砷对动物的毒性作用及中药对其解毒效果。方法用亚砷酸钠毒物染毒小鼠(造模),然后用海尔福口服液(中药制剂)分别按小剂量(治疗1组)和大剂量(治疗2组)灌胃治疗。同时设染毒不治疗组(即模型组)和正常组做对照。实验连续30 d。测定染毒前、后和治疗后的血红蛋白(Hb)。实验结束,从眼球取血,分离血清,测定丙氨酸氨基转移酶(ALT),尿素;处死动物后,取肝脏作病理研究,取大脑制成10%脑匀浆,测定超氧阴离子自由基清除率。结果正常组、模型组、治疗1组、治疗2组,造模前Hb,各组比较(P>0.05)差异无统计学意义;造模后(治疗前)Hb依次分别为143.77±7.11、120.47±14.23、117.66±7.01、125.66±6.86(g/L),正常组明显高于造模的三个组,差异有统计学意义(P<0.01),其中最低为治疗1组,同时造模后的三个组均明显低于其造模前水平,差异有统计学意义(P<0.01)。治疗后Hb分别为152.37±13.69、122.46±12.52、137.62±11.29、151.00±9.09(g/L),模型组最低,明显低于正常组和治疗2组,差异有统计学意义(P<0.05或P<0.01),治疗1组、治疗2组治疗后明显高于治疗前水平,差异有统计学意义(P<0.01),而模型组治疗前后比较,差异无统计学意义(P>0.05)。病理研究结果表明,砷对肝脏有损害作用,模型组肝损害较严重,治疗组较轻。脑对超氧阴离子自由基清除率和肝ALT活性,各组间也有一定变化。结论砷对造血系统有明显的抑制作用和对肝脏有明显损害作用,中药海尔福治疗后恢复明显。

Objective To explore the role of arsenic toxicity to animals and detoxification effects of traditional Chinese medicine.Methods Using sodium arsenide toxicant to contaminate mice（mice model）,and then gave mice gastric lavage treatment with Haier Fu oral liquid（traditional Chinese medicine）.The mice were divided into group 1 with small dose of Haier Fu oral liquid and group 2 with large dose.Moreover,set groups of poisoned without treatment（model group 1） and normal（control） group.The experiment was made for 30 consecutive days.Hemoglobin was tested before and after the exposure and after treatment.At the end of the experiment,drew blood from eyes,separated blood serum and tested alanine aminotransferase（ALT） and carbamide,executed the animals and then made pathological study with liver,took cerebral homogenate and made the homogenate at 10%.The rate of superoxide anion free radical was tested.Results The hemoglobin（Hb）of normal group,model group,treatment group 1 and treatment group 2 were compared before modeling,this difference,however,was not statistically significant（P0.05）.After modeling（before treatment）,hemoglobin were 143.77 ± 7.11,120.47 ± 14.23,117.66± 7.01,125.66 ± 6.86（g / L）,the normal group was significantly higher than that of three model groups（P 0.01）,treatment group 1 got the lowest level,the Hb of three modeling groups was significantly lower than that pro-modeling（P0.01）.After treatment,Hb were 152.37±13.69,122.46±12.52,137.62 ±11.29,151±9.09（g / L） respectively,model groups were the lowest which were significantly lower than that of normal group and treatment group 2（P0.05 or P 0.01）,after the treatment,the Hb of treatment group 1 and treatment group 2 was significantly higher than that before treatment（P0.01）,while those of model group were not significantly changed（P0.05）.The result of pathological study showed that arsenic has damage effect on liver,model groups damaged comparatively serious and then were treatment groups.Conclusion Arsenic has obvious inhibitory effect on hematopoietic system and has damage effect on liver,while the function recovers significantly after treatment with Haier Fu oral liquid.