中国近海角木叶鲽(Pleuronichthys cornutus)种群遗传多样性研究

Genetic diversity of four Pleuronichthys cornutus populations in coastal waters of China

测定了中国沿海角木叶鲽4个群体57个体的线粒体控制区序列,在长度为549 bp的线粒体控制区序列中,检测到3个插入/缺失位点,24个变异位点(包括11个简约信息位点),共出现了37个单倍型,其中9个为共享单倍型。角木叶鲽整体的单倍型多样度(Hd)和核苷酸多态度(P)i结果分别为0.979±0.008和0.00655±0.0037,呈现出高单倍型多样度和低核苷酸多态度分布模式。中性检验结果显示,角木叶鲽种群的Fu's Fs为显著负值,核苷酸不配对分析呈现单峰分布,表明角木叶鲽在历史上经历过种群扩张事件。群体内个体间的平均遗传距离为0.0065,群体间遗传分化指数都为负值(-0.0067^-0.0460)但不显著(P>0.1),AMOVA分析显示遗传变异主要集中在群体内个体间(102.15%),均表明我国近海角木叶鲽种群无明显的分化,可以作为一个渔业管理单位加以保护和利用。

The length of 549 bp mtDNA control region sequences of 57 individuals from 4 Pleuronichthys cornutus populations in the coastal waters of China was determined. 3 insert/deletion sites, 24 variable sites （including 11 parsinomy-informative sites） and 37 haplotypes were detected, of which 9 haplotypes were shared among populations. The global haplotype diversity （Hd） and nucleotide diversity （P/） were 0.979：1：0.008 and 0.0061＋0.0037 respectively, indicating a high haplotype diversity （Hd） and a low nucleotide diversity （P/）. The significant negative values of neutral test of Fu＇s Fs and the unimodal mismatch distribution revealed a historical population expansion. As the average genetic distances within 4 populations were 0.0065, and genetic differentiation index （Fst） were negative （-0.0067^-0.0460） but not significant （P 〉 0.1 ＂~. and l^he m^ority genetic variation （102.15%） occurred within populations （102.15%） in AMOVA analysis~, suggesting that Pleuronichthys cornutus populations in the coastal waters of China were not significantly differentiated and might be protected as a single management unit.