抗体技术研究进展(1):人源抗体技术

Recent advances in antibody technique(1):Humanized antibody technique

100年来,抗体的发现为人类疾病诊断、治疗和有害物质的分析检测发挥了巨大的作用.特别是1975年发明了单克隆抗体技术以及1986年发明基因工程抗体技术,为研制特异性高、大量均一并大量生产抗体成为了现实,也使嵌合抗体、全人源抗体造福人类并产生巨大的经济效益.为了克服鼠源性单抗可诱发人抗鼠抗体(HA-MA),通过嵌合抗体、改构抗体、小分子抗体等技术和改良抗体与抗原结合的特异性,已成为抗体技术研究的主要发展方向,本文主要就抗体人源化及抗体分子小型化,抗体功能复合化两个部分的进展进行综述.

In the past 100 and treatment and the analysis antibody years, antibody has played a significant role in human disease diagnosis of detrimental substances. Especially, the inventions of both monoclonal technique in 1975 and genetic engineering technique in 1986, on one hand, have made it possible for producing abundant antibodies of high specificity and homogeneity, on the other eric antibody and fully human antibody bring benefit to human beings. To overcome the hand, help chimproblem that mufine monoclonal antibody may induce HAMA, technologies such as chimeric antibody, reshaping antibody, small-molecule antibody and improvements of the specificity between antibody and antigen have become the main trend when developing antibody technique. This review gives an overview on antibody humanization, small-molecule antibody and composite function of antibody.