乳头状甲状腺癌VDR及OPN的表达与钙化的关系及临床意义

The Relationship between the VDR and OPN Expressions and Calcification in Papillary Thyroid Cancer and Clinical Significance

通过检测甲状腺良恶性病灶中维生素D受体(vitamin D receptor,VDR)及骨桥蛋白(Osteopontin,OPN)的表达情况,探讨其在甲状腺癌钙化及发生、发展中的作用。实验组选取钙化和非钙化各30例的乳头状甲状腺癌病例共60例,对照组用良性结节性甲状腺肿15例和甲状腺腺瘤15例,通过免疫组织化学法检测良恶性甲状腺组织中VDR及OPN的表达情况,分析其在良恶性之间、乳头状甲状腺癌钙化与非钙化之间以及其他临床病理因素之间的差异及相关性。结果VDR与OPN在乳头状甲状腺癌中的表达明显高于良性病灶(P<0.01);VDR与OPN的表达与患者性别、年龄及淋巴结转移之间无统计学差异,但与乳头状甲状腺癌是否钙化及大小之间有统计学差异,并存在正相关性。肿块钙化或大于1cm的乳头状甲状腺癌VDR和OPN的表达明显增高,VDR和OPN表达亦为正相关(P<0.05)。因此,VDR和OPN蛋白增强可能参与了乳头状甲状腺癌钙化的形成以及肿瘤发生、发展的过程。

By the detection of the vitamin D receptor （ VDR） and osteopontin protein （ OPN） expressions in thyroid benign and malignant lesions, to explore its role in the calcification and occurrence and development of thyroid cancer. Select a total of 60 cases of calcified and non-calcified 30 cases of papillary thyroid cancer cases for experimental group, in the con trol group with 15 cases of benign nodular goiter and 15 cases of thyroid adenoma. VDR and OPN expressions were detec ted by immunohistochemistry in benign and malignant thyroid tissue. The difference and correlation of VDR and OPN ex pressions were analyses between benign and malignant, between the papillary thyroid cancer calcification and non--calcified, and other clinicopathological factors. VDR and OPN expressions in papillary thyroid cancer was significantly higher than benign lesions （P〈0.01）. No significant difference existed between the expressions of VDR and OPN and patient gender, age and lymph node metastasis, but there were significant differences in papillary thyroid cancer whether calcifica tion and size, and there were positive correlation. VDR and OPN expressions were significantly increased in tumor calcification, or greater than lcm of papillary thyroid cancer, and VDR and OPN expressions were also positive correlation （P〈 0.05 ）. VDR and OPN enhancement may be involved in the calcification of papillary thyroid carcinoma formation and cancer development process.