3种离子液体与甲霜灵二元混合物的联合毒性

Joint toxicities of three binary mixture between metalaxyl and ionic liquid

选择3种咪唑类离子液体(ILs):C10H19ClN2(IL1),C12H23ClN2(IL2),C16H31ClN2(IL3)和一种杀菌剂甲霜灵(MET)为混合物组分,以直接均分射线法构建3组二元混合物体系:MET-IL1,MET-IL2和MET-IL3.应用微板毒性分析法(MTA)测定二元混合物对青海弧菌Q67(Vibrio qinghaiensis sp.–Q67)的联合毒性.通过比较实验毒性数据与浓度加和(CA)参考模型分析混合物的毒性相互作用,并利用半数效应浓度(EC50)水平下的等效线图分析毒性变化规律.结果表明3组二元混合物的相互作用明显不同.在MET-IL1和MET-IL2 2组二元体系中,MET浓度比例越高,拮抗作用越明显;在MET-IL3二元体系中,随着MET浓度比例的减小,MET与IL3的相互作用由加和变为协同,并且MET比例越小,协同作用越明显.

Selecting three imidazolium-based ionic liquids（ILs）,C10H19ClN2（IL1）,C12H23ClN2（IL2）,and C16H31ClN2（IL3）,and metalaxyl（MET） as the mixture components,three groups of binary mixture,MET-IL1,MET-IL2,and MET-IL3,were designed by using the direct equipartition ray design procedure.The toxicities of the individual chemicals and binary mixtures to Vibrio qinghaiensis sp.–Q67 were determined by the microplate toxicity analysis（MTA）.The toxicity interaction was evaluated by comparing the toxicity observed to that predicted by the concentration addition（CA） model and employing the isobologram at median effect concentration（EC50）.The toxicity interactions of three groups of binary mixtures were significantly different.In the binary mixtures of MET-IL1 and MET-IL2,the higher the concentration ratio of MET was,the more obvious the antagonism.However,the toxicity interaction in the MET-IL3 mixtures was additive for the high concentration ratio of MET and synergistic for the low concentration ratio of MET.The lower the ratio of MET was,the stronger the synergistic action was.