期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

脑胶质瘤细胞系U251中Nanog基因表达和细胞耐药及侵袭力的关系 被引量：1

下载PDF
导出
摘要 目的探讨细胞耐药后干细胞因子Nanog表达及侵袭力的改变。方法脑胶质瘤细胞系U251卡莫司汀干预后通过RT-PCR检测干预前后细胞中Nanog在mRNA的水平表达情况,通过Transwell体外侵袭实验检测卡莫司汀干预前后侵袭力的改变。结果脑胶质瘤细胞系U251卡莫司汀干预5d后干细胞基因Nanog mRNA表达增高,卡莫司汀干预后穿膜细胞数高于干预前的细胞数(P<0.05)。结论卡莫司汀只能杀死普通肿瘤细胞,而肿瘤干细胞却相对增加,这是生长增殖、耐药和侵袭转移的主要原因。
作者 安玉胜
机构地区 山东省临沂市肿瘤医院头颈外科
出处 《军医进修学院学报》 CAS 2012年第11期1164-1166,共3页 Academic Journal of Pla Postgraduate Medical School
关键词 脑胶质瘤 NANOG 卡莫司汀 耐化疗药
分类号 R34 [医药卫生—基础医学]
  • 相关文献

参考文献15

  • 1Reya T, Morrison SJ, Clarke MF, et al. Stem cells, cancer, and cancer stem cells [ J . Nature, 2001, 414 ( 6859 ) : 105-111.
  • 2Ho MM, Ng AV, Lam S, et al. Side population in human lung Cancer cell lines and tumors is enriched with stem-like Cancer cells [ J ] . Cancer Res, 2007, 67 (10) : 4827-4833.
  • 3Singh SK, Hawkins C, Clarke ID, et al. Identification of human brain tumour initiating cells [ J ] . Nature, 2004, 432 ( 7015 ) : 396-401.
  • 4邓永文,方加胜,李茗初,陈风华,刘劲芳,伍军,周向阳,方芳,卢明,陈成,周仁辉,曾飞跃.胶质瘤中肿瘤干细胞的分离、培养及鉴定[J].中国现代医学杂志,2005,15(16):2449-2452. 被引量：16
  • 5Dean M, Fojo T, Bates S. Tumour stem cells and drug resistance[ J ]. Nat Rev Cancer, 2005, 5 (4) : 275-284.
  • 6Mitsui K, Tokuzawa Y, Itoh I-I, et a|. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells [ J ]. Cell, 2003, 113 ( 5 ) : 631-642.
  • 7Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells [ J ]. Cell, 2003, 113 ( 5 ) : 643-655.
  • 8Hart AH, Hartley L, Parker K, et al. The pluripotency homeobox gene NANOG is expressed in human germ cell tumors [ J ] . Cancer, 2005, 104 ( 10 ) : 2092-2098.
  • 9You JS, Kang JK, Seo Dong-wan, et al. Depletion of embryonic stem cell signature by histone deaeetylase inhibitor in NCCIT cells : involvement of Nanog suppression [ J ] . Cancer Res, 2009,69 ( 14 ): 5716-5725.
  • 10Ezeh UI, Turek PJ, Reijo RA, et al. Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma [ J ] . Cancer, 2005, 104 ( 10 ) : 2255-2265.

二级参考文献34

  • 1李茗初,陈风华,邓永文,伍军,周仁辉,卢明,陈成,周向阳,方芳,方加胜.悬浮法培养C6胶质瘤细胞系和该细胞系中脑肿瘤干细胞的分离[J].中国现代医学杂志,2004,14(24):57-60. 被引量：20
  • 2Huntly BJ,Gilliland DG.Cancer biology:summing up cancer stem cells[J].Nature,2005,435(7046):1169-1170.
  • 3Mitsui K,Tokuzawa Y,Itoh H,et al.The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells[J].Cell,2003,113(5):631-642.
  • 4Zhang S,Balch C,Chan MW,et al.Identification and characterization of ovarian cancer-initiating cells from primary human tumors[J].Cancer Res,2008,68(11):4311-4320.
  • 5Takaishi S,Okumura T,Tu SP,et al.Identification of Gastric Cancer Stem Cells Using the Cell Surface Marker CD44[J].Stem Cells,2009,27(5):1006-1020.
  • 6Singh SK,Hawkins C,Clarke ID,et al.Identification of human brain tumour initiating cells[J].Nature,2004,432(7015):396-401.
  • 7Chambers I,Colby D,Robertson M,et al.Functional expression cloning of Nanog,a pluripotency sustaining factor in embryonic stem cells[J].Cell,2003,113(5):643-655.
  • 8Hart AH,Hartley L,Parker K,et al.The pluripotency homeobox gene NANOG is expressed in human germ cell tumors[J].Cancer,2005,104(10):2092-2098.
  • 9Siu MK,Wong ES,Chan HY,et al.Overexpression of NANOG in gestational trophoblastic diseases:effect on apoptosis,cell invasion,and clinical outcome[J].Am J Pathol,2008,173(4):1165-1172.
  • 10Jeter CR,Badeaux M,Choy G,et al.Functional evidence that the self-renewal gene NANOG regulates human tumor development[J].Stem Cells,2009,27(5):993-1005.

共引文献31

同被引文献19

引证文献1

二级引证文献6

军医进修学院学报
2012年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部