酸枣仁提取物配五味子提取物组方对小鼠睡眠的影响

Effects of spina date seed extract,schisandra chinens P.E compound on the hypnotic of mice

目的探讨酸枣仁提取物配五味子提取物(以下简称"酸枣仁五味子提取物")组方对小鼠睡眠的影响。方法将50只ICR小鼠随机分为正常对照组、酸枣仁五味子提取物组方(0.075g/kg、0.15g/kg、0.3g/kg)组和枣仁安神胶囊(0.45g/kg)阳性对照组。连续灌胃给药7天后,通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验、巴比妥钠睡眠潜伏期实验,观察各组对小鼠睡眠的影响。结果酸枣仁五味子提取物组方灌胃给药后对小鼠无直接睡眠作用;与正常对照组比,酸枣仁五味子提取物组方低、中、高剂量组能显著性延长戊巴比妥钠诱导的小鼠睡眠时间(P<0.05,P<0.01);能显著增加戊巴比妥钠阈下剂量诱导小鼠睡眠发生率,与阈下剂量戊巴比妥钠有协调催眠作用(P<0.05,P<0.01);能有效缩短小鼠巴比妥钠睡眠潜伏期(P<0.05,P<0.01)。结论酸枣仁五味子提取物组方具有明显的镇静、催眠作用,可有效地改善睡眠。

Objective To study the effects of spina date seed extract , schisandra chinens P. E compound on the hypnotic of mice. Methods 50 ICR mice were randomly divided into normal control group,spina date seed extract, schisandra chinens P. E compound（O. 075 g/kg,0. 15 g/kg,0. 3 g/kg） group and zaoren sedative capsule （0.45 g/kg）group. After giving drugs via oral gavage for seven days,the effects of the hypnotic of mice was studied by the experiments of sleep-induced directly, prolonging sleeping time induced by pentobarbital sodium, affecting mice sleeping rate under the threshold hypnogenesis dosage of pentobarbital sodium and affecting sleeping delitescence induced by tobarbital sodium. Results Spina date seed extract, schisandra chinens P. E compound had not sleep-induced directly whereas the compounds could significance prolong sleeping time induced by pentobarbital sodium（P〈0. 05, P〈0.01 ）, increased the incidence rate of sleeping induced by subthreshold dose pentobarbital sodium（P〈0. 05,P〈0.01 ） and shorten sleeping delitescence induced by tobarbital sodium （ P 〈0. 05, P 〈0.01 ）. Conclusion Spina date seed extract , schisandra chinens P. E compound have obvious sedative and hypnotic effects and can improve sleeping efficiently.