全自动免疫组化染色仪在抗肝纤维化中的应用研究

The application of immunohistochemistry autostainer in anti-hepatic fibrosis

目的初步研究全自动免疫组化染色仪在临床病理工作中的应用。方法选取2012年1～4月使用姜黄素治疗的肝纤维化的小鼠12只,分为观察组和对照组。观察组6只小鼠肝脏组织使用全自动免疫组化染色仪进行免疫组化检测,对照组6只小鼠肝脏组织使用手工方法进行免疫组化检测,统计两组免疫组化切片着色均匀度、定位准确度、背景清晰度、单位组织面积抗体使用量、染色时间来评价两种免疫组化方法。结果观察组6只小鼠组织切片着色均匀5例,定位准确6例和背景清晰6例,均显著高于对照组(P均<0.05)。观察组抗体使用量为1.2 ng/cm2,平均染色时间15 min,均显著低于对照组(P均<0.05)。结论全自动免疫组化染色仪具有着色均匀、定位准确、背景清晰、节约抗体和节省时间等特点,是适合病理研究人员和操作人员使用的现代仪器。

Objective To discuss the application of immunohistochemistry autostainer in anti-hepatic fibrosis. Methods 12 liver fibrosis mice with curcumin treatment collected from January 2012 to April 2012 were divided into observation group and the control group. Immunohistochemistry autostainer was used to stain 6 mice liver tissues of observation groups and manual method was used to stain 6 mice liver tissues of control group. Coloring uniformity, positioning accuracy,the back- ground definition, antibody usage and staining time were used to evaluate the effect of immunohistochemistry autostainer used in immunohistochemistry. Results There were 5 cases of coloring uniformity,6 cases of accurate positioning and 6 cases of clear background,which were significantly more than the control group（P 〈 0.05）. Antibody usage was 1.2 ng/cm2 and the average staining time was 15 min,which were significantly lower than the control group（P 〈 0.05）. Conclusion Immunohistoehemistry autostainer with the. advantages of uniform coloring, accurate positioning, clear background,antibod- ies saving and staining time saving is suitable for pathology researchers and operations staffs to use.