摘要
目的探讨PDCD4基因对小鼠T淋巴细胞亚群分化的影响。方法采用流式细胞术检测PDCD4基因缺失小鼠脾细胞和淋巴结细胞中的CD8+T和CD4+T,及其亚群Th1、Th2、Th17和调节性T细胞(Treg)的特异性标记分子,并分析各细胞亚群的比例变化。结果 pdcd4-/-小鼠脾脏和淋巴结中CD8+T细胞数量较野生型C57BL/6小鼠有所下降(P<0.05),CD4+T细胞及Th1细胞也呈下降趋势,差异无统计学意义(P>0.05);而PDCD4基因缺失后Th2和Th17的分化比例无显著变化(P>0.05);进一步分析CD4+T细胞群体中CD25+Foxp3+Treg的表达,发现pdcd4-/-小鼠CD25+Foxp3+Treg比例明显上调(P<0.05)。结论 PDCD4基因能够影响部分T淋巴细胞亚群的分化,PDCD4基因敲除小鼠中CD8+T细胞数量下降和CD25+Foxp3+Treg比例升高,提示PDCD4基因有可能在免疫调节方面起到一定作用。而PDCD4基因对于CD4+T以及Th1、Th2和Th17的分化并无明显作用。
Objective To investigate the influence of PDCD4 gene to the differentiation of T lymphocyte subsets.Methods Detect the expression differences of CD8＋T,CD4＋T and its subsets such as Th1、Th2,Th17 and CD25＋Foxp3＋Treg in the splenocytes and lymph nodes of pdcd4-/-mice and C57BL/6 mice by flow cytometry.And analyse the ratio change of the lymphocyte subsets.Results Compared with that of the C57BL/6 mice,the number of CD8＋T cells decreased in the pdcd4-/-mice（P0.05）.CD4＋T and Th1 cells also showed a descending tendency but had no statistic significance（P0.05）.The proportion of CD4＋CD25＋Foxp3＋Treg significantly increased in the pdcd4-/-mice（P0.05）.Conclusion PDCD4 can influence the differentiation of partial T lymphocyte subsets,the low expression of CD8＋T cells as well as high proportion of CD4＋CD25＋Foxp3＋Treg in the pdcd4-/-mice suggest that PDCD4 gene may play a role in the immune regulation.However,PDCD4 gene has no significant influence on the differentiation of CD4＋T and Th1、Th2 and Th17 cells.
出处
《山东大学学报（医学版）》
CAS
北大核心
2012年第11期6-10,17,共6页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金(81172863)
