整合素-β1在转化生长因子-β2诱导晶状体上皮细胞转化中的作用

Integrin β1 is involved in trans-differentiation of len epithelial cells induced by transforming growth factor-β2 in vitro

目的探讨整合素-β1(integrinβ1)在转化生长因子-β2(TGF-β2)体外诱导晶状体上皮细胞(HLECs)向肌成纤维细胞转化中的作用。方法体外培养的HLECs,选择传3代的细胞进行实验,以TGF-β2作为诱导剂。免疫荧光方法检测HLECs integrinβ1、E-cadherin、α-SMA和F-actin的表达,RT-PCR方法检测E-cadherin和α-SMAmRNA的表达。其中部分细胞预先加入去整合素kistrin孵育1h,再加入TGF-β2刺激细胞48h。结果 TGF-β2处理晶状体上皮细胞后,integrinβ1、α-SMA和F-actin的表达明显增强,细胞E-cadherin表达明显减弱。加入去整合素kistrin后,细胞α-SMA和F-actin的表达明显减弱,细胞E-cadherin表达明显增强。结论 TGF-β2诱导了HLECs转分化,整合素-β1参与了TGF-β2诱导的HLECs向肌成纤维细胞的转化过程。

Objective To investigate the effect of integrin β1 in trans-differentiation of human len epithelial cells（HLECs） induced by transforming growth factor-β2（TGF-β2） in vitro.Methods HLECs were cultured and passaged.The 3rd generation of HLECs were treated with TGF-β2.Expressions of integrin β1,E-cadherin,α-SMA and F-actin in the cells were analyzed with immunofluorescent staining.Expressions of α-SMA mRNA and E-cadherin mRNA were detected by RT-PCR.For some cells,disintegrin kistrin was pre-treated 1h followed by TGF-β2 incubation in 48 h.Results Expressions of integrin β1,α-SMA and F-actin were significantly increased after treatment of TGF-β2,while expression of E-cadherin was significantly decreased after treatment of TGF-β2.Disintegrin kistrin partly reversed TGF-β2 induced expressions o f a-SMA,F-actin and E-cadherin.Conclusion TGF-β2 induced trans-differentiation of HLECs towards myofibroblasts.Integrin β1 was involved in initiation of the trans-differentiation induced by TGF-β2.