黄芪联合顺铂对喉癌Hep-2细胞的体外抑制作用

Inhibitory effect of cisplatin combined with Astragalus on human laryngeal carcinoma Hep-2 cells in-duced by

目的研究黄芪与顺铂联合对体外培养的人喉癌Hep-2细胞的抑制作用。方法用MTT法检测黄芪、顺铂单独或联合作用对人喉癌Hep-2细胞的抑制率；用流式细胞技术及Western．blotting法检测黄芪、顺铂单独或联合作用对人喉癌Hep-2细胞凋亡的影响。结果作用24h后黄芪组、顺铂组、黄芪联合顺铂组对Hep-2细胞的抑制率分别为（53．83±17．12）％、（69．12±27．12）％、（84．55±27．84）％，与对照组（O％）比较差异有统计学意义（t=16．87，16．67，40．90，P〈0．01）。黄芪组、顺铂组、黄芪联合顺铂组Hep-2细胞的凋亡率[（38．2±13：6）％、（67．2±17．8）％、（86．4±25．1）％]与对照组[（17．1±1．3）％]比较差异有统计学意义（t=8．11，12．77，24．92，P〈0．05），且联合用药组的效果较任一单独用药组更为明显（t=11．33，9．37，P〈0．01）。结论黄芪通过促进细胞凋亡有效提高顺铂对Hep-2细胞抑制作用的敏感性，通过调节Bcl-2／Bax蛋白表达，能提高顺铂对喉癌的治疗效果。

Objective To study the anti-proliferation and apoptosis induction effect of cisplatin combined with Astragalus on human laryngeal cancer Hep-2 cells. Methods The proliferation inhibition of cisplatin and Astragalus alone or in combination on Laryngeal cancer Hep-2cells was measured by MTF as- say. Effects of cisplatin and Astragalus alone or in combination on apoptosis of Hep-2 cells were detected by flow cytometry （FCM）. Western blot was used to analyze the expressions of Bcl-2 and Bax proteins. Re- sults The inhibition ratio of proliferation of Hep-2 cells was （53.83 ± 17. 12） % in the astragalus group, （69. 12 ± 27.12）% in the cisplatin group, and （84. 55 ± 27.84）% in the cisplatin combined with Astragalus group, and was significantly greater than the control group （0%） （ t = 16. 87, 16. 67,40. 90, P 〈 0. 01 ）, respectively. The apoptotic ratio of Hep-2 cells was （38.2 ± 13.6）% in the astragalus group, （67.2±17.8 ） % in the cisplatin group, and （ 86.4 ± 25.1 ） %] in the cisplatin combined with Astragalus group, and was significantly greater than control group （17. 1±1.3 ）% （ t = 8. 11,12. 77, 24. 92, P 〈 0.05）, respectively. The effect in the combination group is better than the other group （ t = 11.33,9. 37, P 〈 0. 01 ）. The expressions of Bcl-2 and Bax proteins were changed. Conclusions The tumor-killing effect of cisplatin on laryngeal cancer Hep-2 cells could be enhanced significantly by the combination appli- cation of astragalus by the way of regulating the expression of Bcl-2/Bax.