HPV感染与乳腺癌及其癌组织C-erbB-2表达的关系

Relations of HPV infection with breast cancer and C-erbB-2 expression in breast cancer tissues

目的:研究人乳头瘤病毒(HPV)感染与乳腺浸润性导管癌(IDC)患者临床病理特征的关系及其癌组织中癌基因C-erbB-2表达的相关性。方法:用表面等离子谐振法(SPR)检测60例IDC组织和30例乳腺纤维瘤组织以及30例正常乳腺组织HPV DNA及分型;免疫组化法检测IDC组织中C-erbB-2蛋白的表达。结果:IDC组织HPV感染率为36.7%(22/60),其中HPV 33占其阳性总数的59.1%(13/22),乳腺纤维瘤组织与正常乳腺组织HPV感染率分别为13.30%和3.33%,明显低于IDC组织(χ2=5.300,P=0.021);IDC组织HPV感染与患者组织学分级、腋窝淋巴结转移有关(均P<0.05);IDC组织中HPV感染和C-erbB-2蛋白的阳性表达呈正相关(r=0.486;P<0.001)。结论:HPV感染在IDC发病中起了重要作用,且与肿瘤的分化程度及侵袭转移能力密切相关;HPV感染可能通过促进C-erbB-2的高表达而参与乳腺癌的发生发展。

Objective： To investigate the relations of human papillomavirus （HPV） infection with the clinicopathologic characteristics of invasive ductal carcinoma （IDC） patients and its correlation with the proto-oncogene c-erbB-2 expression in the tumor tissues of the patients. Methods： HPV DNA detection and typing were performed by surface plasmon resonance （SPK） in the specimens of IDC breast tissues （60 cases）, fibroadenoma breast tissues （30 cases） and normal breast tissues （30 cases）. Meanwile, the C-erbB-2 protein expression in the IDC breast tissues was detected by immunohistochemical staining. Results： the FIPV infection rate in IDC breast tissues was 36.7% （22/60）, in which the HPV-33 accounted for 59.1% of the total HPV positive cases （13/22）. The HPV infection rate in fibroadenoma breast tissues and normal breast tissues group was 13.30% and 3.33% respectively, which was significantly lower than that in IDC breast tissues （~=5.300, P=0.021）. HPV infection in the IDC tissues was associated with the histologic grade and axillary lymph node metastasis of the patients （both P〈0.05）. There was a positive correlation between HPV infection and C-erbB-2 protein expression in IDC breast tissues （r=-0.486, P〈0.001）. Conclusion： HPV infection contributes significantly to the pathogenesis of IDC, and also closely related to the degree of differentiation and invasive ability of the tumor. HPV infection participates in the occurrence and development of breast cancer and that may probably be effected by promoting the high expression of C-erbB-2.