儿童型与成人型脑干胶质瘤差异性的相关miRNA表达谱初步研究

Preliminary study on aberrant expression of miRNAs related to the formation of the distinction between pediatric and adult types of brainstem gliomas

目的筛选儿童型脑干胶质瘤（BSG）对照于成人型BSG差异表达的miRNA，为探索儿童型BSG发病机制和治疗中的miRNA干预策略提供可供选择的目标miRNA。方法利用miRNA芯片检NJL童型与成人型BSG原位动物模型的miRNA表达谱，分析二者差异性表达的miRNA，并挑选差异表达最明显的miRNA在两种BSG的人体标本中进行qRT-PCR和原位杂交验证。结果两个组别中共检测到216个miRNA表达，以成人型BSG为比照，儿童型BSG中3．5倍以上差异表达上调的miRNA10个、下调的29个。其中差异最明显的为miR-20a和miR-106b，选取二者在两种BSG的人体标本中进行qRT—PCR和原位杂交验证，结果提示芯片结果适用于人类。结论研究两种BSG的差异性表达的miRNA，将会为解释儿童型BSG较之成人型BSG的恶性进展及以此为控制BSG这-严重威胁儿童生命的疾病提供新的思路。

Objectives To study the different expression of miRNA between pediatric and adult types of brainstem gliomas, and to provide the target miRNAs for explore the mechanism and miRNA interference of the malignant progression of pediatric BSG. Methods miRNA expression profiles in orthotopic models which could simulate the BSG heterogeneity were examined by microarray and analyzed to obtain the aberrantly expressed miRNAs. The two types of human BSG tissue were utilized to verify the microarray data by qRT-PCR and in situ hybridization for the putative causative miRNAs. Results There were 216 miRNAs detected in both the pediatric BSG group and the adult BSG group, 39 miRNAs to be differential expressed in the pediatric BSG group versus adult group, including 10 up-regulated and 29 down- regulated, qRT-PCR and in situ hybridization indicated good consistency with that of the microarray method. Conclusions Aberrantly expressed miRNA may serve as putative causative involvement of malignant progression of pediatric BSG, thereby might be potentially novel targets for therapy.