大鼠腰椎管狭窄模型的制作及三维有限元分析

Three-dimensional finite element analysis and establishment of the lumbar spinal stenosis model in the rats

背景:腰椎管狭窄模型的制作方法有很多种,硅胶植入造成椎管狭窄跟人体退变造成的椎管狭窄结果相似。目的:采用硅胶片植入大鼠椎管内建立腰椎管狭窄大鼠模型,用平板运动距离测量和三维有限元软件分析论证。方法:30只雄性SD大鼠随机分为两组。将硅胶片植入实验组L4和L5阶段硬膜外间隙,造成椎管狭窄。对照组处理与实验组操作过程相同,但不植入硅胶片。结果与结论:实验组平板运动距离较造模前明显减少(P<0.05);脊髓压迫50%-70%。对照组正常椎管内密度CT值为(32.4±19.58)Hu,实验组相应狭窄阶段椎管内CT值为(101.96±32.85)Hu。结果表明,大鼠腰椎管狭窄模型制作成功,制作方法简单,经济有效。

BACKGROUND： The establishments of lumbar spinal stenosis model are variable, and the results of spinal stenosis caused by silicone implant are similar to which caused by degeneration of the human body. OBJECTIVE： To establish the lumbar spinal stenosis model using silicon chip to implant spinal canal in the rats, and to analyze the model through measuring the walking function and three-dimensional finite element. METHODS： Thirty male Sprague Dawley rats were divided into two groups. Two pieces of silicone rubber were implanted into the lumbar （L4 and L5） epidural space to cause spinal stenosis in experimental group. Operation processes were the same in both groups, but silicone rubber was not implanted in the control group. RESULTS AND CONCLUSION： In the experimental group, the postoperative running distance was significantly decreased as compared with the preoperation （P 0.05）, and the spinal was compressed about 50%-70%. The density of normal spinal canal was （32.4±19.58） Hu in the control group, while in the experimental group was （101.96±32.85） Hu. The results showed that the rat models of lumbar spinal stenosis are successfully established, and the making method is simple, effective and economical.