冬虫夏草经肿瘤滋养动脉插管注入对兔VX2肝癌模型的疗效研究

Study on the effect of transcatheter super-selective tumor-feeding intraarterial infusion of rabbit implanted VX2 hepatic tumor by cordyceps sinensis

目的评价冬虫夏草经肿瘤滋养动脉注入对兔VX2肝癌模型肿瘤组织生长抑制及肿瘤转移的影响。方法将40只成功建立为VX2肝癌模型的大白兔随机均分成A、B、C、D 4组:超液态碘油组(A组)、冬虫夏草组(B组)、冬虫夏草加超液态碘油组(C组)、生理盐水对照组(D组)。每组模型在肿瘤移植后14d进行肝动脉插管造影及相关药物超选择性肿瘤滋养动脉内注入,药物注入后7d进行CT检查及病理学检查。结果实验前A、B、C、D组肿瘤体积分别为(387.5±157.3)、(401.1±164.9)、(375.1±154.3)、(375.1±184.0)mm3,各组比较差异无统计学意义(F=0.255,P=0.855);实验后各组肿瘤体积分别为(922.6±32.9)、(685.8±97.9)、(352.5±171.8)、(1 403.5±409.2)mm3,各组比较差异有统计学意义(F=26.13,P=0.030);各组肝内转移分别为:6、4、1、10例(P<0.05);腹腔淋巴结转移分别为:5、2、0、8例(P<0.05)。结论冬虫夏草经肿瘤滋养动脉注入对兔VX2肝癌模型的肿瘤组织生长有抑制作用,能明显减少肿瘤肝内及远处转移的发生率。

Objectlve To evaluate the influence of growth inhibition and metastases of the hepatic tumor tissue of rabbit implan- ted VX2 hepatic tumor via transcatheter super-selective tumor feeding intraarterial infusion by cordyceps sinensis. Methods 40 rab- bits bearing VX2 liver tumor were randomly divided into four groups and each group contained 10 rabbits：lipoidol group（group A）, cordyceps sinensis（group B）,cordyceps sinensis-lipoidol group（group C）,normal saline group（group D）. After implant 14 d,each group model underwent hepatic arterial digital subtraction angiography（DSA）and via transcatheter super-selective tumor-feeding in- traarterial infusion,after 7 d of experimental study,spiral CT scan and histopathology examination were done. Results The tumor volumes before therapy（V1） were（387.5±157.3）,（401, 1±164.9）, （375.1± 154.3）,（375.1±184.0）ram3 respectively,without differences among them（F=0. 255,P=0. 855）. But the tumor volumes after TACE（V2） were（922.6±32.9）,（685.8±97.9）, （352.5±171.8） ,（1 403.5±409.2）mm2 respectively（F=26.13,P=0. 030 4）. Metastases in hepatic each group were found in 6, 4,1,10 cases（P〈0. 05）,celiac lymphonodus metastases in each were found in 5,2,0,8 cases（P〈0. 05）. Conclusion The cordyceps sinensis has effective treatment for the tumor tissue and it＇s metastases with VX2 rabbit liver cancer via transcatheter su- per-selective tumor-feeding intarterial infusion.