孟鲁司特对大鼠脊髓损伤保护作用的研究

The study on protective effect of montelukast on spinal cord injury in rats

目的观察孟鲁司特对大鼠脊髓损伤(SCI)的保护作用,以及对星形胶质细胞反应性增生的抑制作用。方法健康SD大鼠54只,采用Allen法在T9造成急性SCI模型。随机分为孟鲁司特治疗组(A组)、对照组(B组)和甲泼尼松龙组(C组),每组18只。伤后1、3、7d分别用BBB评分和斜板试验观察大鼠运动功能的恢复情况;分别测定血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)浓度,观察自由基变化;用HE染色进行正常神经元计数,采用免疫组织化学检测胶质原纤维酸性蛋白(GFAP)和IL-1β的表达。结果大鼠SCI后3、7dBBB评分和斜板试验A组明显优于B组(P<0.05);大鼠SCI后A组1、3、7dMDA浓度明显低于B组(P<0.05),A组SOD活性明显高于B组(P<0.05)。脊髓损伤后A组GFAP和IL-1β的表达以及正常神经元计数明显优于B组(P<0.05)。结论大鼠脊髓损伤后早期使用孟鲁司特,能有效抑制脊髓损伤后的脂质过氧化反应、星形胶质细胞反应性增生和炎症反应,对脊髓损伤具有保护作用。

Objective To observe the protective effect of montelukast on spinal cord injury（SCI） in rats and the inhibition of re- active hyperplasia of astrocytes. Methods 54 healthy rats were randomly divided into group A,B and C,and SCI was made by A1- lenrs mode（10 g× 25 mm） on Spinal cord T9 extradually. Each group had twelve rats. At the 1 d,3 d and 7 d after SCI,the recovery of motor function after spinal cord injury in rats was assessed with Basso-Beattie-Bresnahan（BBB）scale and slanting board test,Su- peroxide dismutase（SOD）activity and mabnaldehyde（MDA）level in the plasma were measured, and was observed the change of free radicals. HE staining was used for normal neuron counting and immunohistochemical staining detected the expression of GFAP and IL-1β. Results BBB scores and slanting board test of group A were better than those of group B,which had significant difference at 3 d and 7 d after SCI（P〈0.05）. After 1,3,7 d of SCI in rats,concentration of malondialdehyde of group A was lower than that of group B（P〈0.05）. SOD activity of group A was higher than that of group B, which had significant difference after injury（P〈 0.05）. The normal neuron counting, expression of GFAP and IL-1β of group A was better than that of group B,which had signifi- cant difference after 1,3,7 d of SCI（P〈0.05）. Conclusion Early montelukast can inhibit the lipid peroxidation,reactive hyperpla- sia of astrocytes and inflammatory reaction after spinal cord injury in rats. It has a protective effect on spinal cord injury.