天山北坡棉铃虫遗传多样性的RAPD分析

Random Amplified Polymorphic DNA Analysis of the Genetic Diversity of Helicoverpa armigera(H(u|¨)bner) on the Northern Slope of Tianshan Mountains

【目的】对采自乌鲁木齐、三坪农场和阜康222团的棉铃虫从分子水平上进行遗传多样性分析。【方法】利用RAPD(Random Amplified Polymorphic DNA)技术。【结果】筛选出11条引物,并利用这些引物对棉铃虫的5个种群进行遗传分化的研究。222团番茄种群棉铃虫遗传多样性水平最高,乌鲁木齐玉米种群其次,昌吉南瓜种群最低。【结论】玉米、苘麻和棉花三种不同寄主间,棉铃虫基因交流更频繁;同阜康相比,昌吉和乌鲁木齐两个地理位置的棉铃虫更易进行基因交流。

[ Objective] This paper aimed to analyze the genetic diversity of Helicoverpa armigera （Htibner） that came from Urumqi and Fukang on the level of DNA molecule. [ Method] 11 RAPD primers were used for analyzing genetic diversity of 5 species of cotton bollworm. [ Result ] H. armigera had the highest levels of genetic diversity that lived on the 222th fanning regiment ; H. armigera had high levels of genetic diversity that fed on UYM. The lowest genetic diversity were described in H. armigera fed on CJNG. [ Conclusion] Relatively high levels of migration and small genetic distance were characterized between H. armigera of corn, piemarker and cotton; compared with that from Fukang, high levels of migration were characterized between H. armigera from Changji and Urumqi.