岩舒对人大肠癌细胞株HCT8/VCR多药耐药的逆转作用及P糖蛋白表达的影响

REVERSAL OF MULTIDRUG RESISTANCE AND EXPRESSION OF P-GP ON HUMAN COLORECTAL CANCER CELL LINE HCT-8/VCR BY YANSU

目的研究岩舒对大肠癌耐药细胞株HCT-8/VCR的逆转作用及P糖蛋白(P-gp)表达的影响。方法 MTT法测定岩舒含药血清对HCT-8/VCR细胞株的逆转作用,并以流式细胞仪测定P-gp的表达。结果经岩舒含药血清作用72 h后,HCT-8/VCR细胞株对化疗的耐药性显著下降,对长春新碱逆转指数为0.893±0.085,其逆转倍数大于13.7倍,与维拉帕米比较无统计学差异(p>0.05)。HCT-8/VCR细胞的P-gp表达显著高于HCT-8(p<0.01),经岩舒含药血清作用后P-gp表达显著降低(p<0.01)。结论岩舒可以逆转人大肠癌耐药细胞株HCT-8/VCR的多药耐药性,其机制可能与其下调P-gp的表达有关。

Objective To investigate the reversal effect of Yansu on muhidrug resistance of human colorectal cancer cell line HCT - 8/VCR and the impact of Yansu to the expressions of P - gp in HCT - 8/VCR. Methods The reversal effect of Yansu was observed by MTY assay, and the expressions of P - gp in HCT - 8/VCR cell line was detected with flow cytometry （FCM）. Results After stimuhaneous treatment for 72 hours withYansu, the sensi- tivity of HCT -8/VCR rose significantly to VCR. The reversal index （RI）to VCR was 0. 893 ± 0. 085, and there are no statistical significance compared with VER group （p 〉0. 05）. The P -gp had higher expression in HCT -8/VCR than in HCT - 8 （p 〈 0.01 ）. It showed significant decrease after Yansu treatment （p 〈 0. 01 ）. Conclusion Yansu can reverse the resistance of HCT - 8/VCR cel 1 1 ine. The mechanism may be related to the effect of down - regulation the expression of P - gp.