莫氏兰的组织培养与快速繁殖

Tissue Culture and Rapid Propagation of Mokara

以腋芽为外植体,建立莫氏兰的组织培养和再生体系。结果表明:腋芽采用0.1%升汞消毒2次的效果最好;6-BA 4.0 mg/L+NAA 0.4 mg/L的激素配比适合原球茎诱导;原球茎增殖培养基的最佳6-BA、NAA浓度分别是1.0、0.1 mg/L;不定芽诱导培养基的最适6-BA、NAA浓度分别是0.5、0.05 mg/L;在生根培养基VW(含NAA 0.5 mg/L,香蕉40 g/L,土豆30 g/L,糖20 g/L,卡拉胶8.0 g/L,活性炭1.5 g/L)上幼苗生根率达到100%;移栽成活率达95%。

The axillary buds of Mokara was used as explants to establish a tissue culture and regeneration system. The results indicated that axillary buds is suitable to sterilized twice mode with 0.1% HgC12. 6-BA 4.0 mg/L ＋NAA 0.4 mg/L was good for inducing the protoeorm-like bodies. Multiplication medium of 6-BA 1.0 mg/L ＋NAA 0.1 mg/L was the best. 6-BA 0.5 mg/L-4-NAA 0.05 mg/L was the optimum concentration for adventitious bud forming. It was on rooting medium （VW＋NAA 0.5 mg/L ＋coconut water 100 m//L＋banana 40 g/L＋potato 30 g/L＋sucrose 20 g/L＋carrageenan 8.0 g/L＋AC 1.5 g/L） that the rooting rate found was 100%. The transplantation surviving rate could reach 95%.