卡瓦胡椒素B对人非小细胞肺癌A549细胞的抑制作用

Inhibitory Effect of Flavokawain B on Human Non-small Cell Lung Cancer A549 Cells

卡瓦胡椒素B是药用植物卡瓦胡椒根中的一种天然查耳酮类化合物,研究了其对人非小细胞肺癌A549细胞的增殖抑制作用及其诱导细胞凋亡的分子机制.实验结果显示,卡瓦胡椒素B能显著抑制非小细胞肺癌A549细胞的增殖,且随着药物浓度的增加、处理时间的延长其抑制作用呈明显的剂量时间效应;同时,卡瓦胡椒素B能显著诱导A549细胞凋亡、细胞周期阻滞于G2-M期;分子机制研究表明,卡瓦胡椒素B能通过活化JNK激酶活性、下调凋亡抑制蛋白survivin的表达以及激活PARP活性从而导致其对A549细胞的增殖抑制作用.结果表明卡瓦胡椒素B对人非小细胞肺癌的预防与治疗可能具有潜在价值.

The anti-lung tumor potential of flavokawain B,one of active chalcones isolated from Kawa was investigated.Flavokawain B’s action was assessed on proliferation,apoptosis,cell cycle and molecular mechanisms in human non-small cell lung cancer（NSCLC） A549 cells in vitro.The results demonstrated that flavokawain B significantly inhibited the growth of A549 cells in a dose-and time-dependent manner.Meanwhile,flavokawain B induced cell apoptosis and cell cycle G2-M phase arrest in A549 cells.Mechanistically,flavokawain B could activate JNK signaling pathway,down-regulate the expression of survivin protein,and activate the cleavage of PARP,leading to marked inhibitory effect on A549 cells.These findings suggest that flavokawain B may be a potential usefulness for preventing and treatment of NSCLC.