熊珍膏对大鼠CRP及TNF-ɑ的调节作用及对皮肤创面愈合影响的实验研究

Accommodation of Xiongzhen Ointment on CRP,TNF-ɑ and the Effects on Skin Wound Healing of Rats

目的:观察熊珍膏在大鼠皮肤创面愈合过程中对大鼠血清CRP及TNF-ɑ的干预情况,探讨其抗炎及促进创面愈合的作用机理。方法:建立大鼠皮肤创面模型后分为4组,模型组、贝复济对照组(西药组)、熊珍膏组(中药组)、熊珍膏+贝复济组(中西组)各18只。各创面给予相应药物;每日定时换药2次。于造模后第3、7、14天取材,采用双抗体夹心ABC-ELISA法检测大鼠血清中CRP变化情况;采用双抗体夹心ABC-ELISA法检测大鼠血清TNF-ɑ变化情况。实验所得数据均采用-x±s表示,所有结果采用SPSS18.0统计软件进行统计学处理。结果:熊珍膏可以显著降低模型大鼠血清CRP水平,且与中西组无差异;熊珍膏可以显著降低模型大鼠血清TNF-ɑ水平,且与中西组无差异。结论:熊珍膏促进大鼠皮肤切口创面的愈合其机制可能与降低了CRP及TNF-ɑ水平,减少炎症反应有关。

Objective ： To observe the effect of Xiongzhen Ointment in micro-vessels and number of fibroblasts in rat cutane- ous incised wound healing and investigate the mechanism of accelerating the rat back wound healing. Methods： After setting up the model, the rats were randomly assigned to 4 groups： model group, bFGF-ESSEX group （western medicine control group）, Xiongzhen Ointment group, bFGF-ESSEX ＋ Xiongzhen Ointment group. The medicine were attach to the skin twice a day. After setting up models for 3, 7and 14 days, the rats were killed to perform immune-histochemistry study. S-P method of immune-his- tochemistry was used to detect CD34, number of fibroblasts of the local granulation tissue. We used image analysis equipment and computer software to analyze the image of each group. The data were expressed as Mean＋SD and was analyzed by SPSS 18. 0. Results ： The number intensity of granulation tissue were significantly better in Xiongzhen Ointment group than model group in all three time points （P〈O. O1 ）. The improvement of number of fibroblasts was significantly better in Xiongzhen ointment group than model group in all three time points. Conclusion： The Xiongzhen Ointment can accelerate the healing of skin trauma of the rats and improve the quality of healing. This may be related to microvessels improvement and fibroblasts numbers of granulation tissue.