提高和稳定肝素钠产率的措施研究

Efficient Process of Crude Heparin Sodium Production

研究了提高肝素钠产率的主要工艺条件 ,肝素钠生产中 4种提高收率的方法结果表明 :(1)酶解提取比盐解增加收率 12 .1% ,酶、盐解二次提取提高 11.1%～ 19.2 % ;(2 )二次吸附提高收率 15.1% ;(3)二、三次洗脱分别增加 2 0 .7%和 4 .4 % ;(4 )粗产品精制处理。加入Na2 SO3和沉淀剂YNB 99- 1收率可达 98.1%。 1种稳定产率的措施是逐月增加树脂用量和添加乙醇再生处理树脂。

Not only heparin hydration,resin absorption,elution,crude treatment but also ether the usage and treatment of resin D-254 were reported in this paper.The collecting ratios,12.1%,11.1%～19.2%,15.1% as well as 20.7%and4.4% were increasing in the processes of protease hydration,the second hydration,the second resin absorption as well as the second and the third elution of NaCl.While Na 2SO 4 and precipitate YNB 99-1 were utilized for conservation,the collectiong ratio of heparin activity was up to 98.1% in HCl treatment which made heparin potency become to more than 90 USP u/mg.For guaranteeing the stability of heparin collectiong ratio,the quantity of used D-254 resin had to be increased month by month and treated with ethanol.