宁夏干旱荒漠区苦豆子内生放线菌生态分布及抑菌活性研究

Studies on the distribition of endophytic actinomyces and antimicrobal activity from Sophora alopecuroides of arid and desolate areas in Ningxia

从宁夏灵武白芨滩国家级自然保护区不同土壤类型、不同优势植被的6个样区采集的健康苦豆子中,分离得到153株内生放线菌.同一植株不同组织中,内生放线菌数量以根部最多,其次为种子,叶部最少;淡灰钙土中苦豆子内生放线菌数量比风沙土中多.鉴定结果表明:苦豆子内生放线菌以链霉菌属(Stredtomvc)和诺卡氏菌属(Nocardia)较多;通过皿内对峙培养,对153株苦豆子内生放线菌进行抑菌活性测定,拮抗菌株数占总菌株数的90.2%,个别菌株抑菌带宽度达到20mm以上.

The health Sophora alopecuroides which separated 153 endophytic actinomyces were collected from 6 different sample of different soil types and different preponderant vegetations in Baijitan national nature reserve in Ningxia. In different originations of same plant, the number in root was the most endophytic actinomyces, then in stems, and the least in leaves. The number of endophytic actinomyces from S. alopecuroides, which in sandy soil is more than in sandy soil. The result shows that the Streptomyces and Nocardia were the main genera of endophytic actinomyces. By confrontation training in dish, the antimicrobal activity was tested on 153 S. alopecuroides＇s endophytic actinomyces, the antagonistic strains number of 6 sample areas were 90. 2%, the inhibition zone of individual strains reached up to 20 ram.