血细胞分析仪两种模式检测结果比对分析和偏倚评估

Result comparison and bias analysis of two kinds of hematocyte analysers

目的通过对BC-1800血细胞分析仪全血模式和预稀释模式进行方法比对和预期偏倚评估,探讨两种模式之间检测结果是否具有可比性,或检测结果的偏差是否在允许范围内。方法依据美国国家临床实验室标准化协会EP9-A2文件要求,以全血模式为比较方法,预稀释模式为实验方法,对白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)和血小板(PLT)进行方法学比对、相关性分析和预期偏倚估计,判断偏倚是否可以接受。结果所有项目均具有良好的相关性(r≥0.75),预期偏差均未超出允许偏差范围,偏倚可以接受。结论在严格按照操作规程做好质控的基础上,BC-1800血细胞分析仪两种模式对WBC、RBC、Hb和PLT的检测结果具有可比性。

Objective To investigate the comparison of test results on the determination or mode whole blood and mode pre-dilution obtained from Sysmex BC-1800 and expected results bias with acceptable range.Methods According to the United States National Clinical Laboratory Standards Institute（CLSI） EP9-A2,mode whole blood as the comparative method and mode pre-dilution was used for experimental method,a method-comparison and correction analysis and expected bias for WBC,RBC,Hb and PLT were detected to determine deviation within acceptable range.Results The results were closely correlated（r0.75）,and results bias was within the acceptable range.Conclusion In according with the rules to do the control of quality,results of WBC,RBC,Hb and PLT obtained from two kinds of mode of Sysmex BC-1800 is comparable.