阴道分泌物的细菌检查方法探讨

Exploration of vaginal secretions about bacteria check method

目的探讨妇产科阴道分泌物细菌检查的实验室检查方法,为阴道炎患者提供准确可靠的诊断依据。方法对昆明市第一人民医院门诊15 810例18～50岁已婚妇女的阴道分泌物进行生化快速酶法和革兰染色显微镜检查法。结果在15 810例妇女中细菌性阴道炎生化快速酶法占总例数的31.0%;其中唾液酸苷酶法占8.8%;β-葡萄糖醛酸苷酶法占11.0%;凝固酶法占11.2%。革兰染色显微镜检测法阳性占总例数的31.9%。革兰染色显微镜检查法分别与上述3种酶法检测对比的阳性率分别为9.0%、11.6%和11.3%。革兰染色镜检法分别与唾液酸苷酶法、β-葡萄糖醛酸苷酶法、凝固酶法相比较差异无统计学意义(P>0.05)。结论阴道分泌物细菌检查采用生化快速酶法与革兰染色显微镜检查法可以同时检测相互补充,以提高阴道分泌物细菌检查的准确率和阳性率。

Objective To explore gynecology and obstetrics vaginal bacteria check laboratory examination method,and provide accurate and reliable vaginitis diagnosis.Methods In our hospital service,15810 female outpatients aged from 18 to 50 were detected by fast enzymatic and tissue gram stain microscope methods about vaginal secretions.Results In the 15810 cases,bacterial vaginitis biochemical fast enzymatic were 31% of the total number.One saliva acid enzymatic were 8.8%.Beta acid enzymatic glucose were 11%,Solidification enzymatic accounted for 11.2%.Positive rate of Gram′s staining method of total number microscope was 31.9%.Gram stain had no significant difference with anti-bma saliva acid enzymatic nucleoside,beta acid enzymatic,solidification glucose（P0.05）.Conclusion Vaginal bacteria can be detected by biochemical fast enzymatic method and Gram stain microscope,which can improve the accuracy of vaginal bacteria check and test.